Response of Fenugreek (Trigonella foenum-graecum L.) Genotypes for their Germination and early Seedling Parameters Grown Under Different Saline levels

Abstract

Seedling establishment is a most critical stage that determined the crop production at later stages. An in vitro investigation was carried out at the Laboratory of the Department of Plant Breeding and Genetics, Sri Karan Narendra College of Agriculture, Jobner (Rajasthan) in Rabi season 2016-17 to study the response of fenugreek genotypes for their germination and early seedling parameters under five salt stress levels viz. 0.0 mM, 40 mM, 80 mM, 120 mM and 160 mM. Fifteen seeds of each genotype were sown in sterilized Petridish and seedling observations were recorded. Results revealed that the average value was found maximum in the control and minimum at higher salinity level for most of traits. Higher reduction was found at higher salinity levels for most of the characters viz., plumule length, radicle length, seedling length, plumule to radicle length ratio, plumule fresh weight, radicle fresh weight, plumule dry weight, radicle dry weight and seedling vigour index but germination percentage showed less reduction. The contrast between mean of various genotypes in control  conditions (S0) versus Sm (mean of S1, S2, S3 and S4) for each character also showed that overall mean was highest in control as compared to the overall mean of salinity levels for all the traits. This investigation concluded that higher level of salinity (above 60 mM) adversely most affected to seed germination and other early seeding traits rather than (below 40 mM) lower salinity levels. Based upon the rank totals of genotype over different salinity levels S1, S2, S3 and S4 (Sm) and characters, the RMt-303 was found to be most desirable genotype followed by UM-385, RMt-143 and RMt-305. These lines would be used as a viable option under saline conditions and better exploit in hybridization programme.