Pathogenic Bacteria Found on Surfaces of Canned Drinks and Wines Being Sold In Retail Shops in Ondo state, Nigeria, Health Implications, Food Safety and Quality Assessment

Abstract

The aim of this study is to determine the pathogenic bacteria, microbial properties, food safety and quality assessment of canned drinks and wines being sold in retail shops in Ondo state, Nigeria and their   health implications. Bacteria were isolated from canned drinks and Wine Lid, body and bottom surface being sold in retail shops. They were identified using conventional method of analysis. The antibiotic susceptibility (Antibiogram) tests were determined on isolates using disc diffusion method. After the inoculation of the selected parts (Lid, body and bottom surface), sixteen(16) Gram positive pathogenic bacteria were identified. Bacteria isolates includes Bacillus polymyxa (7), Lactobacillus casei, Microbacterium lacticum, Staphylococcus aureus, Cellulomon asbiazotae, Bacillus subtilis, Clostridium sporogens, Staphylococcus pyogens, Bacillus cereus. In this study, Bacillus polymyxa were the most common organism isolated. Some of the Gram positive organisms were resistant to some selected antibiotics. Staphylococcus pyogens were resistant to Norfaxacin, Erythromycin, Gentamycin and Ampliclox. Bacillus polymyxa were resistant to Norfaxacin, Chloramphenicol, and Gentamycin. Lactobacillus casei were resistant to Norfaxacin, Ampliclox and Amoxil. Ultraviolet Spectrophotometer were used to determine the growth dynamic and death rate of the isolates, the addition of antibiotics to the organism at the 48th hour speed up the death rate of the organisms. The result of this study shows that canned drinks and wines top surfaces can harbor pathogenic bacteria, therefore people are encouraged to wash the top surfaces of canned drinks and wines before consumption, to minimize and eliminate the health threat of the isolated pathogenic bacteria in our canned drinks and wine surfaces, this research work will therefore encourage food safety and quality assessment of our canned drinks and wine.