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L-PRF Membrane (Fibrin Rich in Platelets and Leukocytes) and Its Derivatives (A-PRF, i-PRF) are Helpful as a Basis of Stem Cells in Regenerative Injury Treatment: Trial Work on the Horse

Alessandro Crisci, Maria Chiara Barillaro, Giovanni Lepore, Federica Cardillo

International Blood Research & Reviews · pp. 1–14 · Published 22 Feb 2020

10.9734/ibrr/2019/v10i230117

Abstract

Developing a multidisciplinary field of tissue engineering aims to recover, improve or supplant typically damaged or missing tissues for a collection of conditions brought about by trauma, malady and old age. To guarantee that tissue engineering techniques are generally relevant in the clinical setting, it is important to adjust them so that they are promptly accessible and moderately simple to use in the everyday clinical schedule. Consequently, the steps between preparation and application must be limited and improved to make them practical in application. The general objective of creating platelet concentrates of natural origin can be delivered near the patient to quicken the implantation procedure, being monetarily practical for the patient and the health framework. Fibrin rich in platelets and leukocytes (PRF) and its derivatives (L-PRF, A-PRF, i-PRF) has been utilized in a wide collection of medical fields for delicate tissue restoration. Practically all platelets (> 97%) are missing within test-tubes in groups tested after PRF membrane extraction. Growth Factors liberated by platelets contained in derivatives of L-PRF induce and control the proliferation and migration of other cell types, associated with tissue repair, similar to smooth cell muscles (SMCs) and mesenchymal stem cells (MSCs). Conclusively, the outcomes of this work feature the positive effects of PRF on wound healing after regenerative treatment for the administration of different delicate tissue defects found in wound care.

Blood derivatives growth factors injectable platelet-rich fibrin leucocyte platelet-rich fibrin L-PRF wound box stem cells.

References (5)

  1. 1 A proposed protocol for the standardized preparation of PRF membranes for clinical use [DOI]
  2. 2 Use of Leukocyte Platelet (L-PRF) Rich Fibrin in Diabetic Foot Ulcer with Osteomyelitis (Three Clinical Cases Report) [DOI]
  3. 3 Platelet Counts in Insoluble Platelet-Rich Fibrin Clots: A Direct Method for Accurate Determination [DOI]
  4. 4 An Evaluation of the Accuracy of the Subtraction Method Used for Determining Platelet Counts in Advanced Platelet-Rich Fibrin and Concentrated Growth Factor Preparations [DOI]
  5. 5 Temporal release of growth factors from platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) in the horse: a comparative in vitro analysis.

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CLINICAL AND EXPERIMENTAL RATIONALE FOR THE FEASIBILITY OF USING A COMBINED FIBRIN–BONE SCAFFOLD WITH A PRONOUNCED LOCAL ANTIMICROBIAL EFFECT

Yu. I. Yevchuk, A. V. Pantus · Bulletin of Problems Biology and Medicine · 2025

COMPARATIVE ANALYSIS OF THE RESULTS OF COMBINED MINERALISED AND DEMINERALISED FIBRIN-BONE SCAFFOLDS USE IN SINUS-LIFT TECHNIQUE

A. O. Cheliy, A. V. Pantus, M. M. Rozhko · Bulletin of Problems Biology and Medicine · 2025

Quantificazione delle piastrine e dei leucociti nei concentrati piastrinici solidi del sangue per uso chirurgico: studio di una tecnica sperimentale

Alessandro CRISCI, Tomoyuki KAWASE, Raffaele D’ADAMO · Chirurgia · 2021

THE EFFECTIVENESS OF USING A COMBINED FIBRIN-BONE SCAFFOLD FOR BONE TISSUE REPAIR IN JAW DEFECTS

Yu. I. Yevchuk · Bulletin of Problems Biology and Medicine · 2025

Analisi citomorfologica di fibrina ricca di piastrine liquida prodotta con centrifuga ad angolo fisso DUO (Process, Francia) per l’uso nella terapia rigenerativa delle ulcere cutanee

Michela Crisci, Giovanni Lepore, Federica Feleppa · Italian Journal of Wound Care · 2023

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