DPPH and ABTS Radical Scavenging Assay and Hemolytic Potential of Methanol Leaf Extract of Erythrina crista-galli L.

Abstract

Background: Erythrina crista-galli is a medicinal plant containing bioactive constituents of potential biological importance. Evaluation of its radical-scavenging activity and interaction with red blood cells is important for characterising the antioxidant activity and haemolytic potential of its leaf extract. Aim: The present study aims to investigate the antioxidant activity and haemolytic potential of the methanol leaf extract of Erythrina crista-galli. Method: Anti-radical activity was assessed using the DPPH and ABTS radical scavenging assays, and the haemolytic activity of the methanol leaf extract was evaluated. Results: The methanol leaf extract of E. crista-galli demonstrated DPPH scavenging activity; at extract concentrations of 6.25, 12.5, 25, 50, and 75 µg/mL, the recorded inhibition values were 3.125%, 15.625%, 25.0%, 31.25%, and 37.5%, showing a gradual enhancement in antioxidant potential with increasing concentration. The percentage inhibition of ABTS radicals increased progressively with increasing extract concentration, indicating a concentration-dependent antioxidant effect. Quercetin was used as the standard. The plant extract exhibited a concentration-dependent increase in haemolytic activity. Conclusion: The present findings confirm the existence of mild and remarkable antioxidant activity and significant haemolytic potential in the leaf extract of E. crista-galli, and these data could pave the way for further research.