Justicia secunda Leaf Aqueous Fraction Suppressed NF-κB, TNF-α, IL-6, and COX-2 in Arthritic Rat
Sandra A. Kings-Ogbonna, Godswill N. Anyasor
Journal of Complementary and Alternative Medical Research · pp. 34–48 · Published 5 May 2022
10.9734/jocamr/2022/v17i430340Abstract
Aims: This study evaluated the effect of the aqueous fraction of Justicia secunda Vahl leaves on selected inflammatory markers Tumor necrosis factor alpha (TNF-α), nuclear factor–kappa B (NF-κB), interleukin-6 (IL-6) and cyclooxygenase-2 (COX-2) and its possible mechanism of action. Study Design: J. secunda aqueous leaf fraction (JSALF) was tested on carrageenan, arachidonic and Complete Freund’s Adjuvant (CFA) induced rat paw inflammation models, respectively. Methodology: Ninety male albino rats (Wistar strain) were randomly distributed into 6 groups of 5 rats each. Group I: normal, administered with 1 mL NaCl, Group II: untreated control, no treatment, Group III: administered with 10 mg/kg body weight (b.wt.) diclofenac sodium (reference drug), Group IV, V, and VI: administered with 200, 400 and 600 mg/kg b.wt. J. secunda leaf aqueous fraction (JSALF), respectively. TNF-α, NF-κB, IL-6 and COX-2 levels were analyzed using enzyme-linked immunosorbent assays (ELISA). Gas Chromatography-Mass Spectrometry (GC-MS) analysis was carried out. Results: JSALF treated animals had significantly (P < .05) suppressed carrageenan, arachidonic and CFA-induced paw edema when compared with untreated control animals. Serum levels of TNF-α, NF-κB, IL-6 and COX-2 were significantly reduced (P < .05) in JSALF-treated animals. GC-MS detected 10 suspected anti-inflammatory compounds. Conclusion: Findings from this study indicate that J. secunda aqueous leaf fraction substantially suppressed pro-inflammatory mediators and COX-2 pathways in arthritic rats which justifies its folkloric use in the management of inflammatory diseases.
References (29)
- 1 Inflammation and cancer [DOI]
- 2 The Immunomodulatory and Anti-Inflammatory Role of Polyphenols [DOI]
- 3 Diclofenac: an update on its mechanism of action and safety profile [DOI]
- 4 Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins [DOI]
- 5 Function of Nuclear Factor Kappa B (NF-kB) in Human Diseases-A Review [DOI]
- 6 Molecular and Cellular Pathways Contributing to Joint Damage in Rheumatoid Arthritis [DOI]
- 7 Potential of anti-inflammatory agents for treatment of atherosclerosis [DOI]
- 8 Antioxidant, antibacterial, and anti-inflammatory activities of standardized brazilin-richCaesalpinia sappanextract [DOI]
- 9 POLYPHENOLS: METHODS OF EXTRACTION
- 10 Anti-inflammatory Effect of Curcuma longa and Allium hookeri Co-treatment via NF-κB and COX-2 Pathways [DOI]
- 11 Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models [DOI]
- 12 In vitro effects of anthocyanin extracts from Justicia secunda Vahl on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. [DOI]
- 13 Ethnomedical Study and Iron Content of Some Medicinal Herbs Used in Traditional Medicine in Cote D’Ivoire for the Treatment of Anaemia [DOI]
- 14 Scandoside Exerts Anti-Inflammatory Effect Via Suppressing NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages [DOI]
- 15 Rheumatoid arthritis synovial fibroblasts promote TREM-1 expression in monocytes via COX-2/PGE2 pathway [DOI]
- 16 Factors influencing physical activity in patients with early rheumatoid arthritis: A mixed-methods study [DOI]
- 17 The Antimicrobial Screening of a Barbadian Medicinal Plant with Indications for Use in the Treatment of Diabetic Wound Infections [DOI]
- 18 Studies on the anti-inflammatory and analgesic properties of Jatropha curcas leaf extract [DOI]
- 19 In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino rats [DOI]
- 20 In vitro and In vivo Anti-inflammatory Activities of Coptosapelta flavescens Korth Root's Methanol Extract [DOI]
- 21 2,3-Diarylxanthones as Potential Inhibitors of Arachidonic Acid Metabolic Pathways [DOI]
- 22 Investigation of in vivo Analgesic, Anti-Inflammatory, in vitro Membrane Stabilizing and Thrombolytic Activities of Atylosia scarabaeoides and Crotalaria spectabilis Leaves [DOI]
- 23 In Vivo Pharmacological and Anti-inflammatory Evaluation of Xerophyte Plantago sempervirens Crantz [DOI]
- 24 Chemical composition of the leaf and stem essential oil of Adenophorae Radix [DOI]
- 25 Evaluation of the antihypertensive activity of total aqueous extract of Justicia secunda Valh (Acanthaceae) [DOI]
- 26 Secundarellone A, B, and C from the leaves of Justicia secunda VAHL [DOI]
- 27 GC-MS analysis of oil rich in polyenoic fatty acid methyl esters from leaves of Justicia secunda Vahl growing abundantly in the lowland rain forests of the Niger Delta region of Nigeria
- 28 CCR3, CCR5, interleukin 4, and interferon-gamma expression on synovial and peripheral T cells and monocytes in patients with rheumatoid arthritis.
- 29 In Vitro Evaluation of Anti Inflammatory Activity of Methanolic And Ethanolic Leaf Extract of Psidium Guajava [DOI]
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