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Research Article Open access CC BY 4.0

Justicia secunda Leaf Aqueous Fraction Suppressed NF-κB, TNF-α, IL-6, and COX-2 in Arthritic Rat

Sandra A. Kings-Ogbonna, Godswill N. Anyasor

Journal of Complementary and Alternative Medical Research · pp. 34–48 · Published 5 May 2022

10.9734/jocamr/2022/v17i430340

Abstract

Aims: This study evaluated the effect of the aqueous fraction of Justicia secunda Vahl leaves on selected inflammatory markers Tumor necrosis factor alpha (TNF-α), nuclear factor–kappa B (NF-κB), interleukin-6 (IL-6) and cyclooxygenase-2 (COX-2) and its possible mechanism of action. Study Design: J. secunda aqueous leaf fraction (JSALF) was tested on carrageenan, arachidonic and Complete Freund’s Adjuvant (CFA) induced rat paw inflammation models, respectively. Methodology:  Ninety male albino rats (Wistar strain) were randomly distributed into 6 groups of 5 rats each. Group I: normal, administered with 1 mL NaCl, Group II: untreated control, no treatment, Group III: administered with 10 mg/kg body weight (b.wt.) diclofenac sodium (reference drug), Group IV, V, and VI: administered with 200, 400 and 600 mg/kg b.wt. J. secunda leaf aqueous fraction (JSALF), respectively. TNF-α, NF-κB, IL-6 and COX-2 levels were analyzed using enzyme-linked immunosorbent assays (ELISA). Gas Chromatography-Mass Spectrometry (GC-MS) analysis was carried out. Results: JSALF treated animals had significantly (P < .05) suppressed carrageenan, arachidonic and CFA-induced paw edema when compared with untreated control animals. Serum levels of TNF-α, NF-κB, IL-6 and COX-2 were significantly reduced (P < .05) in JSALF-treated animals. GC-MS detected 10 suspected anti-inflammatory compounds. Conclusion: Findings from this study indicate that J. secunda aqueous leaf fraction substantially suppressed pro-inflammatory mediators and COX-2 pathways in arthritic rats which justifies its folkloric use in the management of inflammatory diseases.

Anti-inflammation complete Freund’s adjuvant cyclooxygenase-2 interleukin-6 Justicia secunda

References (29)

  1. 1 Inflammation and cancer [DOI]
  2. 2 The Immunomodulatory and Anti-Inflammatory Role of Polyphenols [DOI]
  3. 3 Diclofenac: an update on its mechanism of action and safety profile [DOI]
  4. 4 Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins [DOI]
  5. 5 Function of Nuclear Factor Kappa B (NF-kB) in Human Diseases-A Review [DOI]
  6. 6 Molecular and Cellular Pathways Contributing to Joint Damage in Rheumatoid Arthritis [DOI]
  7. 7 Potential of anti-inflammatory agents for treatment of atherosclerosis [DOI]
  8. 8 Antioxidant, antibacterial, and anti-inflammatory activities of standardized brazilin-richCaesalpinia sappanextract [DOI]
  9. 9 POLYPHENOLS: METHODS OF EXTRACTION
  10. 10 Anti-inflammatory Effect of Curcuma longa and Allium hookeri Co-treatment via NF-κB and COX-2 Pathways [DOI]
  11. 11 Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models [DOI]
  12. 12 In vitro effects of anthocyanin extracts from Justicia secunda Vahl on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. [DOI]
  13. 13 Ethnomedical Study and Iron Content of Some Medicinal Herbs Used in Traditional Medicine in Cote D’Ivoire for the Treatment of Anaemia [DOI]
  14. 14 Scandoside Exerts Anti-Inflammatory Effect Via Suppressing NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages [DOI]
  15. 15 Rheumatoid arthritis synovial fibroblasts promote TREM-1 expression in monocytes via COX-2/PGE2 pathway [DOI]
  16. 16 Factors influencing physical activity in patients with early rheumatoid arthritis: A mixed-methods study [DOI]
  17. 17 The Antimicrobial Screening of a Barbadian Medicinal Plant with Indications for Use in the Treatment of Diabetic Wound Infections [DOI]
  18. 18 Studies on the anti-inflammatory and analgesic properties of Jatropha curcas leaf extract [DOI]
  19. 19 In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino rats [DOI]
  20. 20 In vitro and In vivo Anti-inflammatory Activities of Coptosapelta flavescens Korth Root's Methanol Extract [DOI]
  21. 21 2,3-Diarylxanthones as Potential Inhibitors of Arachidonic Acid Metabolic Pathways [DOI]
  22. 22 Investigation of in vivo Analgesic, Anti-Inflammatory, in vitro Membrane Stabilizing and Thrombolytic Activities of Atylosia scarabaeoides and Crotalaria spectabilis Leaves [DOI]
  23. 23 In Vivo Pharmacological and Anti-inflammatory Evaluation of Xerophyte Plantago sempervirens Crantz [DOI]
  24. 24 Chemical composition of the leaf and stem essential oil of Adenophorae Radix [DOI]
  25. 25 Evaluation of the antihypertensive activity of total aqueous extract of Justicia secunda Valh (Acanthaceae) [DOI]
  26. 26 Secundarellone A, B, and C from the leaves of Justicia secunda VAHL [DOI]
  27. 27 GC-MS analysis of oil rich in polyenoic fatty acid methyl esters from leaves of Justicia secunda Vahl growing abundantly in the lowland rain forests of the Niger Delta region of Nigeria
  28. 28 CCR3, CCR5, interleukin 4, and interferon-gamma expression on synovial and peripheral T cells and monocytes in patients with rheumatoid arthritis.
  29. 29 In Vitro Evaluation of Anti Inflammatory Activity of Methanolic And Ethanolic Leaf Extract of Psidium Guajava [DOI]

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