Study on the Hypolipidemic Effect of Moringa oleifera Seeds Based on Zebrafish Hyperlipidemia Model

Abstract

Objective: Investigated the hypolipidemic effect of alcoholic extract of Moringa oleifera seeds using the zebrafish hyperlipidemia model. Methods: Using 0.3% egg yolk solution to feed zebrafish larval to construct the hyperlipidemia model. These larval fish in the model were treated with an extract of Moringa oleifera seeds for 2 days. Then, the tail vessels were observed after oil red O staining, and the accumulated optical density of lipids was analyzed. The body length, triglyceride (TG), and cholesterol (TC) levels were measured. Result: The accumulated optical density of lipids in the tail vessels of larval fish fed with 0.3% egg yolk was significantly higher than that of the blank group. The contents of TG and TC were also increased, indicating that the hyperlipidemia model was successfully established. After treatment with different concentrations of Moringa oleifera seed extract (10 μg/ml, 15 μg/ml, and 20 μg/ml), the staining attachment surface of vascular oil red O in the tail of hyperlipidemic zebrafish was significantly reduced by 42.8%, 63.3%, and 80.6% respectively. TG was reduced by 9.89%, 20.41%, and 26.40%. TC was reduced by 9.85%, 23.93%, and 33.80%. The data of each group were statistically significant (P<0.01). Conclusion: Moringa seeds had a significant hypolipidemic effect on zebrafish and had no significant effect on the skeletal growth length of zebrafish.