Screening of Filamentous Fungi for Xylanases and Cellulases Not Inhibited by Xylose and Glucose
L. F. C. Ribeiro, L. F. Ribeiro, J. A. Jorge, M. L. T. M. Polizeli
Biotechnology Journal International · pp. 30–39 · Published 26 Oct 2013
10.9734/BBJ/2014/6066Abstract
Aims: Screening different filamentous fungi for thermostable xylanases and cellulases that would not be inhibited by xylose and glucose, respectively. Methodology: Samples of fungi collected in the Atlantic forest region, Minas Gerais, Brazil, and some fungi from our Culture Collection were used in this screening. All fungi were grown in liquid media containing 1% sugar cane bagasse (SCB). After that, an aliquot of the crude broth was incubated at different temperatures (from 4 to 60 °C) in carboxymethyl cellulose (CMC) or xylan-media plates, for 12 hours. After this period, the plates were stained with Congo Red. Fungi that presented the best results (larger halos) were tested for the effect of adding xylose and glucose in the xylanase and cellulases activities, respectively. Crude extracts obtained from fungi grown in SCB were used for laccase and lichenase assay. Results: The screening on agar plates with CMC/xylan presented halos of different sizes. From all tested fungi, the best cellulase producer was Malbranchea pulchella, which also presented the most thermostable xylanase. Penicillium griseofulvum presented bigger halos at all temperatures tested, but the xylanase lost almost 14% of its stability in higher temperatures. The effect of xylose and glucose on the enzymatic activities recorded dose-dependent. It was observed that 20% activation of the enzymes produced by M. pulchella with 30 mM glucose or 20 mM xylose to cellulase and xylanase, respectively. It was observed a loss of less than 20% for P. griseofulvum xylolytic activity using 50 mM xylose. Lichenase was detected in some fungi prospected but laccase was not detected. Conclusion: Malbranchea pulchella was a good producer of xylanase and cellulase tolerant to xylose and glucose, respectively. Other studies must be performed with this fungus so that it can be used in the future for biotechnological purposes.
Cited by 11
Yuri Heck da Silva, Tássio Brito de Oliveira, Matheus Sanitá Lima · 2022
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, О.M. Yurieva, I.M. Kurchenko · Mikrobiolohichnyi Zhurnal · 2016
Sophie C. Brandt, Bernhard Ellinger, Thuat van Nguyen · PLOS ONE · 2018
Paulo R. Heinen, Jorge H.A. Betini, Maria L.T.M. Polizeli · Reference Module in Life Sciences · 2017
Seema Dahiya, Bijender Singh · 3 Biotech · 2019
Renan do Nascimento Barbosa, José Ewerton Felinto dos Santos, Jadson Diogo Pereira Bezerra · Acta Botanica Brasilica · 2022
Liliane FC Ribeiro, Rosymar C De Lucas, Gabriela L Vitcosque · Biotechnology for Biofuels · 2014
D.K. Zabolotny Institute of Microbiology and Virology of the NASU, Kyiv, Ukraine., Iryna Kurchenko, Olena Yurieva · 2023
Praveen Kumar Reddy Goukanapalle, Dileep Kumar Kanderi, Girinath Rajoji · Cellulose · 2020
Anju Bala, Bijender Singh · World Journal of Microbiology and Biotechnology · 2017
Related research
- Overproduction of Xylanase from Mutants of Bacillus subtilis with Barley Husk as the Prime Carbon Source under Submerged Fermentation after Random Mutagenesis Using Ethyl Methane Sulfonate (EMS) and Acridine Orange (AO) — shares topic coverage
- Strain Development of Aspergillus brasiliensis Using Physical and Chemicals Mutagenesis for Possible Overproduction of Xylanase, Amylase, Protease and Cellulase under Submerged Fermentation (SmF) — shares topic coverage
- Production, Purification and Characterisation of a Purified Low Molecular Weight and Thermo-alkaline Tolerance Xylanase by Aspergillus brasiliensis — shares topic coverage
- Isolation and Identification of Cellulolytic and Xylanolytic Bacteria from Huancarhuaz Hot Spring, Peru — shares topic coverage
- Measurement of Cellulolytic Potential of Cellulase Producing Bacteria — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
11
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.