In vitro UV-Visible and FTIR Spectroscopy Study of Low Power He-Ne Laser Irradiation on Human Blood

Abstract

Laser irradiation has biostimulating effect in various cell types low power laser radiation is used clinically for skin and vascular disorders. Aims: To investigate the effect of (He-Ne) laser (λ= 632nm, power=1mW) on human whole blood, after irradiated to different times from 10 min to 50 min. Study Design:  Human Whole Blood Irradiated to (He-Ne) laser(λ= 632nm, power=1mW).  Place and Duration of Study: Soba Hospital, Khartoum- Sudan, Institute of Laser, Sudan University of science and technology (SUST), February 2018. Methodology: Blood samples were taken from healthy volunteers; blood sample irradiated to (He-Ne) laser and control compared; FTIR and UV-Vis spectrophotometer were used to study laser radiation effect Results: FTIR spectra and UV-vis absorption spectra of blood samples are compared before and after irradiated to He-Ne laser shows increases absorption for 10, and 40 min  but it decreases as the exposure time at 30,20 and 50 minutes. This fluctuation of light absorption is known as a biphasic response. FTIR spectra of non exposed blood showed the peaks due to O-H (free group), C=O (amide I group), N=O (nitro group), and C-H (aromatic group). N-H (Amino acid (amide II). For all exposure time He-Ne laser (λ= 632nm, power=1mW) irradiation, showed significant changes. Increased in transmittance at different exposure time for all groups (C=O, O-H, N=O, C-O & C-H, N-H) and indicates significant decreasing in their concentration. Conclusion: The laser radiation causes changes in the structure and conformational changes in the polypeptide and absorption of blood samples decreased due to increasing ligand electronegativity.