Detection of TEM and SHV Genes in Clinical Escherichia coli and Klebsiella pneumoniae Strains ESBL Isolated in Neonatology and Pediatric Units
V. M’bengue Gbonon, S. A. Afran, K. N. Guessennd, A. A. Toty, T. F. B. Diplo, A. S. P. N’Guetta, M. Dosso
Microbiology Research Journal International · pp. 1–7 · Published 30 Mar 2018
10.9734/MRJI/2018/39354Abstract
Aims: To detect blaTEM and blaSHV genes in Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) strains resistant to β-lactams isolated from neonatal and pediatric infections. Place and Duration: Bacteriology-Virology Department and Molecular Biology platform of Pasteur Institute of Côte d'Ivoire from January 2012 to November 2015. Methods: A total of 38 strains of E. coli and K. pneumoniae ESBL isolated and identified according to classical bacteriology techniques from neonatal and pediatric infections, were subject of our study. Search for blaTEM, and blaSHV genes were carried out by conventional PCR. Results: Molecular research showed that 52.6% of strains were carrying blaTEM gene and blaSHV gene was present in 36.9%. blaTEM and blaSHV genes were present simultaneously in 36.9% of strains. Conclusion: This study revealed a predominance of blaTEM genes in E. coli and K. pneumoniae ESBL strains.
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V. Baldo, C. Salogni, S. Giovannini · Frontiers in Veterinary Science · 2020
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