Isolation, Screening and Identification of Cellulolytic Bacteria from Soil
Biotechnology Journal International · pp. 1–8 · Published 31 Jan 2020
10.9734/bji/2020/v24i130092Abstract
Background: Cellulose is the most abundant carbohydrate on earth and is considered as a good candidate for production of second generation biofuel (ethanol) and many other products of routine use. For degradation, cellulases are used which are mostly secreted by microbes such as fungi. Cellulases also play an important role in senescence of plants and in host-parasite relationship for invading the plant cell wall. However, comparatively lesser studies have been carried out on cellulase producing bacteria. Therefore, present study was aimed to isolate cellulase (Endo-β-1,4-D-glucanase; EC. 3.2.1.4.) from bacterial sources. Methodology: To isolate thermophilic/ mesophilic cellulase producing bacteria, soil samples were collected from wood furnishing area and agricultural farm around Indore. Besides, soil sample was also collected from the vicinity of Amlai Paper Mill in Budhar district, Madhya Pradesh. These soil samples after suitable dilutions were streaked on different nutrients agar petri-dishes having carboxymethyl cellulose (CMC) as an inducer. After screening, four colonies were isolated capable of producing good amount of cellulase. Screening was done using Congo red staining and confirmation was done after growth of the bacteria in liquid nutrient medium having CMC. These colonies individually were grown in suitable nutrient media having CMC as an inducer and enzyme activity was determined in the nutrient media after harvesting bacterial cells by centrifugation. Results: The highest enzyme producing bacteria were identified as Bacillus lichenoformis and Ochrobactrum anthropi after biochemical analyses, 16S rRNA sequencing and subsequently phylogenetic tree analysis.
References (24)
- 1 MUSCLE: multiple sequence alignment with high accuracy and high throughput [DOI]
- 2 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
- 3 Selection of Conserved Blocks from Multiple Alignments for Their Use in Phylogenetic Analysis [DOI]
- 4 Improvement of Phylogenies after Removing Divergent and Ambiguously Aligned Blocks from Protein Sequence Alignments [DOI]
- 5 Microbial Cellulose Utilization: Fundamentals and Biotechnology [DOI]
- 6 Phylogeny.fr: robust phylogenetic analysis for the non-specialist [DOI]
- 7 Cellulases and related enzymes in biotechnology [DOI]
- 8 A Rapid and Easy Method for the Detection of Microbial Cellulases on Agar Plates Using Gram’s Iodine [DOI]
- 9 Extracellular enzyme synthesis in the genus Bacillus [DOI]
- 10 Microbial Cellulose Utilization: Fundamentals and Biotechnology [DOI]
- 11 The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass [DOI]
- 12 Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential [DOI]
- 13 Use of 16S rRNA Gene for Identification of a Broad Range of Clinically Relevant Bacterial Pathogens [DOI]
- 14 Optimization of Cellulase Production from Bacteria Isolated from Soil [DOI]
- 15 Enzymatic properties of cellulases from Humicola insolens [DOI]
- 16 Purification and characterization of carboxymethylcellulase isolated from a marine bacterium, Bacillus subtilis subsp. subtilis A-53 [DOI]
- 17 Cellulases of bacterial origin [DOI]
- 18 Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses [DOI]
- 19 Optimization of Cellulase Production from Newly Isolated Bacillus Sp. Y3 [DOI]
- 20 Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization [DOI]
- 21 Isolation and characterization of a novel Penicillium oxalicum strain Z1-3 with enhanced cellobiohydrolase production using cellulase-hydrolyzed sugarcane bagasse as carbon source [DOI]
- 22 A novel strain of Trichoderma viride shows complete lignocellulolytic activities [DOI]
- 23 Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization [DOI]
- 24 Optimization Studies on Cellulase Production from Bacillus Anthracis and Ochrobactrum Anthropic (YZ1) Isolated from Soil [DOI]
Cited by 22
Debasree Sinha, Sandipan Banerjee, Subhrangshu Mandal · Bioresource Technology · 2021
Majekodunmi Racheal Adedayo, Taofeek Olatunji Lateef · FUDMA JOURNAL OF SCIENCES · 2025
Faculty of Biology - Biotechnology, University of Science, Ho Chi Minh City, VNUHCM, Vietnam, Dung Nguyen, Tuan Tran · Science and Technology Development Journal - Natural Sciences · 2025
Oluwafemi Michael Adedire, Adefoyeke Olufunmilayo Aduramigba-Modupe, Olubusola Ayoola Odeniyi · Journal of Crop Improvement · 2023
Shweta Ashok Bhagat, Seema Sambhaji Kokitkar · Journal of Applied Biology & Biotechnology · 2021
Daniel Menezes-Blackburn, Nahad Al-Mahrouqi, Buthaina Al-Siyabi · Diversity · 2021
Mrunal K. Bhatt, Mousumi B. Das · Journal of Pure and Applied Microbiology · 2024
Showing 7 of 22 known citations — external sources report more than can currently be individually listed.
Related research
- Screening for Non-communicable Diseases in Public Places in Upper Egypt — shares topic coverage
- Breast Cancer Screening Trend Year 2017 among South-Western Nigeria Female Residents — shares topic coverage
- A Statistical Approach towards Cervical Cancer Awareness among Women in Ghana — shares topic coverage
- Screening for Hypertension and Diabetes in an Underserved Population through Community Outreach; A Case of Rural Community in Enugu State, Nigeria — shares topic coverage
- Comparison of the Diagnostic Yields of Some Methods of Screening for Diabetic Peripheral Neuropathy in North Central Nigeria — shares topic coverage
Article metrics
Real usage data collected on this platform.
53
Page views
0
PDF downloads
15
Outbound clicks
22
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.