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Research Article Open access CC BY 3.0

Isolation and 16S rRNA-Based Identification of Benomyl-Degrading Bacteria

Adil A. El-Hussein, Randa H. Elsalahi, Awad G. Osman, Ashraf M. Sherif, Marmar A. El Siddig

Biotechnology Journal International · pp. 670–683 · Published 27 May 2014

10.9734/BBJ/2014/10633

Abstract

Laboratory experiments were conducted to isolate and identify Benlate- (Benomyl) degrading microorganisms from two soil types collected from different locations in Khartoum State, Sudan. Benomyl degradation was studied at two temperatures (28 and 40ºC) in soil treated with three Benomyl concentrations (0.032, 3.2 and 8.0mg Benomyl/g soil) and incubated for 360 days. Potential degraders were also tested in mineral salt liquid medium using Benomyl as a sole carbon source. Degradation percentages were then determined and the most efficient Benomyl degraders were identified by amplification with 16S rRNA gene, sequencing and alignment with deposited sequences in the international gene bank. A total of 64 isolates were recovered from the two soil types, with 59 (92.2%) isolates recovered from the clay soil. Thirty four isolates were recovered from clay soil treated with 8.0mg Benomyl/g soil and incubated at 28ºC. The most efficient Benomyl degraders, with degradation percentages in the range of 44-59, were identified as Pseudomonas stutzeri (Two different isolates), Pseudomonas putida, Acinetobacter johnsonii, Brevibacillus invocatus, Bacillus clausii, Lysinibacillus sp. and Agrobacterium radiobacter.

Benlate MSM Biodegradation clay soil bacteria

References (55)

  1. 1 Modern Soil Microbiology [DOI]
  2. 2 Biodegradation of Low-Density Polyethylene (LDPE) by Mixed Culture of Lysinibacillus xylanilyticus and Aspergillus niger in Soil [DOI]
  3. 3 Biodegradation of Atrazine byAgrobacterium radiobacterJ14a and Use of This Strain in Bioremediation of Contaminated Soil [DOI]
  4. 4 Accessing microbial diversity for bioremediation and environmental restoration [DOI]
  5. 5 Preparation of Bacterial DNA Template by Boiling and Effect of Immunoglobulin G as an Inhibitor in Real-Time PCR for Serum Samples from Patients with Brucellosis [DOI]
  6. 6 Biodegradation of phenol by free and immobilized Acinetobacter sp. strain PD12 [DOI]
  7. 7 Biodegradation of malathion by Acinetobacter johnsonii MA19 and optimization of cometabolism substrates [DOI]
  8. 8 Diversity of chromium-resistant and -reducing bacteria in a chromium-contaminated activated sludge [DOI]
  9. 9 Toluene and styrene removal from air in biofilters [DOI]
  10. 10 Degradation of phenol by Acinetobacter strain isolated from aerobic granules [DOI]
  11. 11 In silico feasibility of novel biodegradation pathways for 1,2,4-trichlorobenzene [DOI]
  12. 12 The Rice Inoculant Strain Alcaligenes faecalis A15 is a Nitrogen-fixing Pseudomonas stutzeri [DOI]
  13. 13 Biodegradation of malathion by Brevibacillus sp. strain KB2 and Bacillus cereus strain PU [DOI]
  14. 14 Biodegradation characteristics of quinoline by Pseudomonas putida [DOI]
  15. 15 Benzene, toluene and xylene biodegradation by Pseudomonas putida CCMI 852 [DOI]
  16. 16 Effect of carbendazim and pencycuron on soil bacterial community [DOI]
  17. 17 Lysinibacillus parviboronicapiens sp. nov., a low-boron-containing bacterium isolated from soil [DOI]
  18. 18 Glyphosate degradation byAgrobacterium radiobacter isolated from activated sludge [DOI]
  19. 19 Pseudomonas azotifigens sp. nov., a novel nitrogen-fixing bacterium isolated from a compost pile [DOI]
  20. 20 Biodegradation of ethanethiol in aqueous medium by a new Lysinibacillus sphaericus strain RG-1 isolated from activated sludge [DOI]
  21. 21 Purification, biochemical properties and substrate specificity of a catechol 1,2‐dioxygenase from a phenol degrading Acinetobacter radioresistens [DOI]
  22. 22 Diversity of the Ring-Cleaving Dioxygenase Gene pcaH in a Salt Marsh Bacterial Community [DOI]
  23. 23 Impact of Pesticide Use on Health in Developing Countries: Proceedings of a Symposium Held in Ottawa, Canada, 17-20 September 1990
  24. 24 Pesticides: Microbial degradation and effects on microorganisms
  25. 25 Isolation of diuron-degrading bacteria from treated soil [DOI]
  26. 26 Biodegradation of gaseous styrene by Brevibacillus sp. using a novel agitating biotrickling filter [DOI]
  27. 27 Bacillus clausii as a Treatment of Small Intestinal Bacterial Overgrowth [DOI]
  28. 28 Biodegradation of Naphthalene byPseudomonas stutzeriin Marine Environments: Testing Cells Entrapment in Calcium Alginate for Use in Water Detoxification [DOI]
  29. 29 Influence of environmental factors on degradation of carbendazim by Bacillus pumilus strain NY97-1 [DOI]
  30. 30 Biodegradation characterisation and kinetics of m-cresol by Lysinibacillus cresolivorans [DOI]
  31. 31 Manual of Microbiology [DOI]
  32. 32 Biodegradation mechanism of biphenyl by a strain ofPseudomonas stutzeri [DOI]
  33. 33 Cometabolism of 3,4-dichlorobenzoate by Acinetobacter sp. strain 4-CB1 [DOI]
  34. 34 Reproductive toxicity of methyl-1-(butylcarbamoyl)-2-benzimidazole carbamate (benomyl) in male wistar rats [DOI]
  35. 35 Involvement of a plasmid in growth on and dispersion of crude oil by Acinetobacter calcoaceticus RA57 [DOI]
  36. 36 Isolation and characterization of a new carbendazim-degrading Ralstonia sp. strain [DOI]
  37. 37 Testing aPseudomonas putidastrain for 4-chlorophenol degradation in the presence of glucose [DOI]
  38. 38 Fate of benomyl on field soil and turf [DOI]
  39. 39 Delayed and Enhanced Degradation of Benomyl and Carbendazim in Disinfested and Fungicide-Treated Soils [DOI]
  40. 40 Characterization of Two Novel Propachlor Degradation Pathways in Two Species of Soil Bacteria [DOI]
  41. 41 Biosurfactants from Acinetobacter calcoaceticus BU03 Enhance the Bioavailability and Biodegradation of Polycyclic Aromatic Hydrocarbons
  42. 42 Biodegradation of Swainsonine by Acinetobacter calcoaceticus strain YLZZ-1 and its isolation and identification [DOI]
  43. 43 Isolation, Molecular and Biochemical Identification of Paraoxon-Metabolizing Pseudomonas Species [DOI]
  44. 44 ISOLATION AND CHARACTERIZATION OF CARBENDAZIM DEGRADING Trichoderma harzianum RIFAI MUTANTS [DOI]
  45. 45 Analysis of Benomyl by Liquid Chromatography/Time-of-Flight Mass Spectrometer and Its Occurrence in the Environment [DOI]
  46. 46 4-Hydroxybenzoate Uptake in an Isolated Soil Acinetobacter sp. [DOI]
  47. 47 Determination of benomyl by reversed-phase liquid chromatography [DOI]
  48. 48 Characterization and growth response of bacteria in soil following application of carbofuran [DOI]
  49. 49 Biodegradation of herbicides bromoxynil, ioxynil and dichlobenil by Agrobacterium radiobacter cells immobilized in polysaccharide matrix [DOI]
  50. 50 Removal of lead from aqueous solution by Acinetobacter calcoaceticus.
  51. 51 Biodegradation of phenol by free and immobilized Acinetobacter sp. strain PD12
  52. 52 Isolation and characterization of Pseudomonas sp. CBW capable of degrading carbendazim [DOI]
  53. 53 Biodegradation of malathion by Acinetobacterjohnsonii MA19 and optimization of cometabolism substrates
  54. 54 Biodegradation of fungicide Thiram (TMTD) in soil under laboratory conditions [DOI]
  55. 55 [Utilization of 3-chlorobenzoic acid by Acinetobacter calcoaceticus].

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