Isolation and Identification of Air Microflora in Clifford University Medical Center, Ihie Campus, Owerrinta, Abia State, Nigeria

Abstract

The settling plate method was used to examine the air microflora in 6 units at the Clifford University Medical Centre, Ihie campus, Owerrinta, Abia state, Nigeria. The male ward, male restroom, female ward, female restroom, theater room, and environment were among the 6 units. At each of the sample locations, the culture plates containing potato dextrose agar and nutrient agar media were exposed to the atmosphere for about 15 minutes. A total count of 11 CFU and 295 CFU fungal and bacterial isolates respectively were identified from the medical center but 5 fungal isolates and 29 bacterial isolates were obtained as pure cultures. These fungi were Aspergillus flavus, Aspergillus niger, Trichophyton sp, Penicillium sp, and white mold. The bacterial genera were Staphylococcus sp, Streptococcus sp, Micrococcus sp, and Bacillus sp. Staphylococcus sp and Aspergillus sp. were the two most prevalent microorganisms in this investigation. It can be said that the Clifford University Medical Centre employed for this study featured numerous types of Gram-positive bacteria, a high prevalence of pathogenic bacteria, and a high prevalence of pathogenic fungi in both the indoor and outdoor air. The majority of the fungal isolates are molds, whereas certain bacterial species are commensals that live on human skin, the fungal isolates are of environmental origin. The bacterial and fungal isolates contained a few non-pathogenic species. The study was carried out to determine if air microfloral affects the infection rate in the University Medical Centre.