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Research Article Open access CC BY 4.0

Phylogenetic Analysis of Bacillus Bacteria from Compost

Ayesha Ameen, Jalil Ahmad, Shahid Raza

Journal of Advances in Biology & Biotechnology · pp. 1–5 · Published 8 Sep 2016

10.9734/JABB/2016/26719

Abstract

Many activated regions of gene can be used to identify the particular organism, this is easier by using the technique of 16S ribotyping of rRNA gene. These regions are present on rRNA gene and are conserved, that is the reason to use them for identification purpose. This study was mainly focused on the phylogenetic analysis of reverse sequence of bacillus strains for 16S rRNA gene. It was concluded that the AB1 and AB2 were closely related and showed direct lineage while the E.coli strain and salmonella showed outgroup relation.  

PCR gel electrophoresis 16S reverse sequence AB1 and AB2

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