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Research Article Open access CC BY 4.0

Investigation of Potential Probiotic Bacterium (Bacillus subtilis CCI3) in the Formulated Diets on Immunity, Growth Performance and Nutritional Quality of Cyprinus carpio

Manisha Ashok Dhotre, Datta Ashok Nalle, Suraj Kadam

Asian Journal of Fisheries and Aquatic Research · pp. 25–42 · Published 27 Jun 2024

10.9734/ajfar/2024/v26i7781

Abstract

In the present investigation Bacillus subtilis CCI3 was evaluated for use as a probiotic supplement in the feeds for the fingerlings of Cyprinus carpio. The outcome of supplement on the feed utilization efficiency, growth performance, and immune response was evaluated.  Cyprinus carpio fingerlings (avg. wt. 27.75 ± 0.045 g) were fed feed supplement with 2 x 104 (feed BS1), 2 x 105 (feed BS2) and 2 x106 (feed BS3) Bacillus subtilis cells per 100g feed for 60 days @ 5 % of the body weight per day in two equal installments in triplicate treatments. The control diet (CC) was not supplemented with the Bacillus subtilis. All the feeds are isocaloric and isonitrogenous. Feed BS2 fed fishes showed better growth, significantly (p ≤ 0.05) higher protein efficiency ratio (PER), highest RNA: DNA ratio and a lower feed conversion ratio (FCR) than the other experimental feeds. Feed BS2 fed fishes also showed highest carcass protein and lipid than the others. Intestinal protease and α – amylase activity was observed significantly higher (p ≤ 0.05) in BS2 feed treated fishes and also significantly (p ≤ 0.05) greatest GPT and GOT values were observed in feed BS2 fed fishes but lowest in control (C). Highest TSP, albumin, globulin was observed in BS2 treated fishes after 60 days feeding trial. But lowest glucose level was observed in the same treatment. TEC, TLC, Hct and Hb values were highest in BS2 treated fishes. After feeding trial the specific and non-specific immunity levels and disease resistance of fish were also studied. NBT, antibody titer and serum bacterial activity were highest in diet BS2 fed fishes. After feeding trial the fishes were challenged for 10 days by bath exposure to Aeromonas sp. (A2) [105 CFU ml-1, 1hr. and after 7 days 107 CFU ml-1, 1hr].

Cyprinus carpio Bacillus subtilis CCI3

References (16)

  1. 1 Lactic acid bacteria in fish: a review [DOI]
  2. 2 Prevention of antibiotic-associated diarrhea by Saccharomyces boulardii: A prospective study [DOI]
  3. 3 Colorimetric Methods of Analysis [DOI]
  4. 4 Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth [DOI]
  5. 5 Practical Haematology [DOI]
  6. 6 Use of Carnobacterium sp. as a probiotic for Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss, Walbaum) [DOI]
  7. 7 Principles of fish nutrition
  8. 8 Effect of a probiotic bacterium Bacillus circulans PB7 in the formulated diets: on growth, nutritional quality and immunity of Catla catla (Ham.) [DOI]
  9. 9 The Benefit of a Roseobacter Species on the Survival of Scallop Larvae [DOI]
  10. 10 Histopathology and cytopathology of white spot syndrome virus (WSSV) in cultured Penaeus monodon from peninsular Malaysia with emphasis on pathogenesis and the mechanism of white spot formation [DOI]
  11. 11 Growth performance, immune-physiological variables and disease resistance of common carp (Cyprinus carpio) orally subjected to different concentrations of Lactobacillus plantarum [DOI]
  12. 12 Probiotic bacteria as growth promoter and biocontrol agent against Aeromonas hydrophila in Catla catla (Hamilton, 1822)
  13. 13 The isolation of intestinal microflora of farmed red seabream (Pagrus major) and black seabream (Acanthopagrus schlegeli) at larval and juvenile stages [DOI]
  14. 14 Water Quality and Growth Performance of Nile Tilapia (Oreochromis niloticus), Chia (Salvia hispanica) and Lemon Grass (Cymbopogon citratus) in a Media-based Aquaponics System [DOI]
  15. 15 A note on the effect of host specific fermented milk on the coliform population of the neonatal rat gut [DOI]
  16. 16 Growth Performance and Muscle Composition of Carps (Labeo rohita, Catla catla, Cirrhinus cirrhosus and Hypophthalmichthys molitrix) at Different Protein Diets under Polyculture Farming [DOI]

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