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Research Article Open access CC BY 4.0

Internal Transcribed Spacer (ITS) Region as Molecular Marker for Parasite Identification in Aquaculture: A Review

Yuniar Mulyani, . Rosidah

Asian Journal of Fisheries and Aquatic Research · pp. 9–17 · Published 17 Aug 2022

10.9734/ajfar/2022/v19i130463

Abstract

Molecular technologies may be quicker and more accurate than the culture, serology, and histology procedures that have historically been employed to identify fish parasites. By using molecular diagnostic methods, parasites can be found in asymptomatic fish, allowing disease outbreaks to be stopped. This review discusses the use of Internal Transcribed Spacer (ITS) markers to detect parasite in aquaculture and their potential applications are discussed. It was found that it is a more reliable marker and species identification in combination with other DNA markers. ITS region are highly polymorphic non - coding region with sufficient taxonomic units. Thus, sequences can be separated at the species level. though ribosomal ITS as a universal barcode marker for parasite identification is still hampered by few limitations, the ITS will remain as the key choice for parasite identification. It remains the most important option for parasite identification. Many studies have been carried out using ITS markers for parasites identification such as cestode, nematodes, Pseudoterranova spp., Anisakis type I, Diplostomum, Gyrodactylids Hysterothylacium sp, Glochidia larvae. This identification of parasites are taken from the species Rainbow trout (Oncorhynchus mykiss), Argentinesea bass, (Acanthistius patachonicus), and other samples from various places.

ITS (Internal Transcribed Spacer) molecular marker parasite aquaculture

References (32)

  1. 1 The its Region of Nuclear Ribosomal DNA: A Valuable Source of Evidence on Angiosperm Phylogeny [DOI]
  2. 2 Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi [DOI]
  3. 3 An evaluation of LSU rDNA D1-D2 sequences for their use in species identification [DOI]
  4. 4 Multiple Hybrid Formation in Natural Populations: Concerted Evolution of the Internal Transcribed Spacer of Nuclear Ribosomal DNA (ITS) in North American Arabis divaricarpa (Brassicaceae) [DOI]
  5. 5 Nuclear ribosomal spacer regions in plant phylogenetics: problems and prospects [DOI]
  6. 6 Heteroplasmy as a common state of mitochondrial genetic information in plants and animals [DOI]
  7. 7 Non-coding nuclear DNA markers in phylogenetic reconstruction [DOI]
  8. 8 Choosing an Approach and an Appropriate Gene for Phylogenetic Analysis [DOI]
  9. 9 Species-Diagnostic Differences in a Ribosomal DNA Internal Transcribed Spacer from the Sibling Species Anopheles Freeborni and Anopheles Hermsi (Diptera:Culicidae) [DOI]
  10. 10 T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives [DOI]
  11. 11 Unraveling the Evolutionary Radiation of the Families of the Zingiberales Using Morphological and Molecular Evidence [DOI]
  12. 12 Effect of frozen period on the chemical, microbiological and sensory quality of frozen tilapia fish ( Sarotherodun galiaenus ) [DOI]
  13. 13 Phylogeny and paraphyly among tetrapod blood flukes (Digenea: Schistosomatidae and Spirorchiidae)☆ [DOI]
  14. 14 Complex Pattern of Coalescence and Fast Evolution of a Mitochondrial rRNA Pseudogene in a Recent Radiation of Tiger Beetles [DOI]
  15. 15 Analyses of the ribosomal internal transcribed spacers (ITS) and the 5.8s gene indicate that extremely high rDNA heterogeneity is a unique feature in the scleractinian coral genus Acropora (Scleractinia; acroporidae)
  16. 16 Molecular identification, morphological characterization and new insights into the ecology of larval Pseudoterranova cattani in fishes from the Argentine coast with its differentiation from the Antarctic species, P. decipiens sp. E (Nematoda: Anisakidae) [DOI]
  17. 17 Identifying freshwater mussels (Unionoida) and parasitic glochidia larvae from host fish gills: a molecular key to the North and Central European species [DOI]
  18. 18 Molecular and morphological evidence for three species of Diplostomum (Digenea: Diplostomidae), parasites of fishes and fish-eating birds in Spain [DOI]
  19. 19 Occurrence and Molecular Identification of Anisakis Dujardin, 1845 from Marine Fish in Southern Makassar Strait, Indonesia [DOI]
  20. 20 Internal Transcribed Spacer (ITS) as Dna Barcoding to Identify Fungal Species: a Review [DOI]
  21. 21 A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region [DOI]
  22. 22 Morpho-molecular characterization of Gyrodactylus parasites of farmed tilapia and their spillover to native fishes in Mexico [DOI]
  23. 23 Identifikasi Bakteri secara Molekular Menggunakan 16S rRNA [DOI]
  24. 24 Structure and organization of ribosomal DNA [DOI]
  25. 25 Molecular Diagnosis of Fish Diseases: a Review
  26. 26 Using the ribosomal internal transcribed spacer (ITS) as a complement marker for species identification of red macroalgae [DOI]
  27. 27 Infection and molecular identification of ascaridoid nematodes from the important marine food fish Japanese threadfin bream Nemipterus japonicus (Bloch) (Perciformes: Nemipteridae) in China [DOI]
  28. 28 Absence of zoonotic parasites in salmonid aquaculture in Denmark: Causes and consequences [DOI]
  29. 29 Molecular basis for identification of species/isolates of gastrointestinal nematode parasites [DOI]
  30. 30 Ribosomal DNA as molecular markers and their applications in the identification of fish parasites (Platyhelminthes: Monogenea) from India [DOI]
  31. 31 Molecular identification of Neoechinorhynchus rutili parasite diagnosed in some fish species caught in Menzelet dam lake in Kahramanmaras province (Turkey) [DOI]
  32. 32 Molecular identification of two cestodes species parasitizing freshwater fishes in India [DOI]

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