Inhibitory Activity of β-lactamases by Hydro-Ethanolic Extracts of Harungana madagascariensis, a Plant of the Ivorian Pharmacopoeia
Toty Abalé Anatole, Aka Ayebe Edwige, Guedé Kipré Bertin, Konan K. Fernique, Adrien Jehaes, N. Guessennd, Otokoré D. Albert, M. Dosso, M. Galleni
Asian Journal of Research in Biochemistry · pp. 157–162 · Published 29 Dec 2020
10.9734/ajrb/2020/v7i430168Abstract
Infection due to multi-resistant bacteria is a public health concern. Unfortunately, the prospect of developing new antibiotics seem not to be on the horizon. Faced with this impasse, medicinal plants could be an alternative for the development of new molecules. The aim of this study was to determine the inhibitory effect of H. madagascariensis extracts on β-lactamases. Extraction of bioactive compounds from trunk barks and leaves of the plant was done in an ethanol-water mixture (70:30). Anti-β-lactamase activity was evaluated by spectrophotometry after the removal of tannins and a phytochemical screening was used to identify the groups of compounds. Concentrations inhibiting 50% of enzyme activity were 0.005±0.001 mg/mL (CTX-M-15), 0.01±0.001 mg/mL (P99) and 0.027±0.009 mg/mL (NDM-1) for bark extracts and 0.704 mg/mL for leaves extracts. Phytochemical screening revealed the presence of flavonoids in bark extracts. The ethanolic extracts of the trunk bark exert a good inhibitory activity on CTX-M-15, P99 and NDM-1 β-lactamases and this activity could be attributed to the presence of the flavonoids. Further studies by bio-guided fractionation of the ethanolic extracts of the bark could yield fractions with high inhibitory potential of β-lactamases.
References (14)
- 1 Plant Products as Antimicrobial Agents [DOI]
- 2 Bad Bugs, No Drugs: No ESKAPE! An Update from the Infectious Diseases Society of America [DOI]
- 3 The Epidemic of Antibiotic-Resistant Infections: A Call to Action for the Medical Community from the Infectious Diseases Society of America [DOI]
- 4 Health and Economic Impacts of Antimicrobial Resistance [DOI]
- 5 Isolation and in vitro antibacterial activity of astilbin, the bioactive flavanone from the leaves of Harungana madagascariensis Lam. ex Poir. (Hypericaceae) [DOI]
- 6 Anti-protozoan activities of Harungana madagascariensis stem bark extract on trichomonads and malaria [DOI]
- 7 Response of Trypanosoma brucei bruceiinduced anaemia to a commercial herbal preparation [DOI]
- 8 Comparative phytochemical and ethnomedicinal survey of selected medicinal plants in Nigeria [DOI]
- 9 Comparative phytochemical and ethnomedicinal survey of selected medicinal plants in Nigeria [DOI]
- 10 Inhibition of Beta-Lactamase by 1,4-Naphthalenedione from the Plant Holoptelea integrifolia [DOI]
- 11 Beta-lactamase inhibitory component from the roots of Fissistigma cavaleriei [DOI]
- 12 Anti-protozoan activities of Harungana madagascariensis stem bark extract on trichomonads and malaria [DOI]
- 13 Beta lactamase Inhibitors from Indigenous Herbs and Spices.
- 14 Phytoalexins: Enzymology and Molecular Biology [DOI]
Cited by 0
No indexed citations yet.
Related research
- Isolation and Identification of Uropathogenic E. coli and its Antimicrobial Susceptibility Pattern with Special Reference to Extended Spectrum Beta-Lactamases (Esbl) — shares topic coverage
- Antibacterial Potential of Aqueous and Hydro-Ethanolic Extracts of Four Plants against Pathogens Present in the Poultry Industry — shares topic coverage
Article metrics
Real usage data collected on this platform.
64
Page views
0
PDF downloads
30
Outbound clicks
0
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.