Induction of Bax and Activation of Caspases by Hydro Ethanolic Leaf Extract of Citrullus colocynthis (L)-mediated Apoptosis in Breast Cancer Cell Line (MCF-7)

Abstract

Cancer is the most deadly disease that causes death among the world. It is caused by genome and epigenome abnormalities. Herbal plants are used for treatment of cancer. Citrullus colocynthis is valuable medicinal plants used for treatment of cancer. The plant as antidiabetic, anticancer activity, and antioxidant activity.The aim of the study is to find induction of bax and activation of caspases by hydro ethanolic leaf extract of Citrullus colocynthis (L)-in Human breast cancer cells (MCF-7). Materials and methods, Human breast cancer cell was purchased from the national centre for cell sciences. Cell line and cell culture were performed and cell viability was done by MTT assay. Total RNA isolation, cDNA conversion and gene expression analysis by real time PCR. This study clearly indicates that plant extract has a significant role in modulation of intrinsic apoptotic signaling in human breast cancer cells which might be due to the presence of cucurbitacin present in Citrullus colocynthis. It is conducted that Citrullus colocynthis extract inhibits the growth of the human breast cancer cells (MCF-7) by regulating the expression of pro apoptotic (Bax) and caspases -3 genes. Hence Citrullus colocynthis may be served as a potential drug for treatment of human breast cancer cells.