Increase in Endoglucanase Productivity and Mycelial Stability of Rhizopus oryzae by Classical Mutagenesis
Sudarshana Bandyopadhyay, Moumita Karmakar, Rina Rani Ray
Biotechnology Journal International · pp. 60–72 · Published 3 Apr 2012
10.9734/BBJ/2012/1037Abstract
Aim: To develop a mutant strain with high endoglucanase productivity and optimization of some cultivation parameters. Place and Duration of Study: Microbiology Research Laboratory, Department of Zoology, Molecular Biology & Genetics, Presidency University, College Street, Kolkata: 700 073, India, between Aug, 2010 and March 2011. Methodology: The wild strain of Rhizopus oryzae PR7 MTCC 9642 was subjected to classical mutagenesis by suspending 5 hyphal discs (0.5 cm) in 10ml of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) solutions of various concentrations (125-1000µg). The in situ cellulolytic activity of the colonies of the mutant strains on the plates were measured by using alcoholic iodine solution and the highest enzyme producing mutant was selected. The mutant strain was later cultivated in presence of various domestic wastes at various pH, temperature, time. The morphological alteration was also checked by staining with fluorescent dye. Results: Out of 50 mutants, strain A7 was selected that showed about 33% increase in endoglucanase synthesis utilizing orange bagasse as sole carbon source in a shake flask screen. The strain was found to have the same pH and temperature optima, but could achieve highest level of enzyme production earlier than that by its wild counterpart. Being a dimorphic fungus, the wild type strain of Rhizopus oryzae, showed a transformation to yeast like pelleted form, whereas the mutant strain A 7 showed persistent filamentous structure indicating the achievement of a structural stability in presence of environmental stress. Conclusion: The present mutant strain could ferment orange bagasse and showed an increased production of endoglucanase with minimized time consumption with greater mycelial stability against various environmental stresses. These achievements will definitely add economy in industrial production of endoglucanse at a nominal cost.
References (29)
- 1 A Rapid and Easy Method for the Detection of Microbial Cellulases on Agar Plates Using Gram’s Iodine [DOI]
- 2 Cellulose degrading enzymes and their potential industrial applications [DOI]
- 3 Directed evolution of enzyme catalysts [DOI]
- 4 Nutrient effects on production of cellulolytic enzymes by Aspergillus niger [DOI]
- 5 Improved Cellulase Production by Trichoderma reesei RUT C30 under SSF Through Process Optimization [DOI]
- 6 Novel mutation method for increased cellulase production [DOI]
- 7 Purification and characterization of new endo-1,4-β-d-glucanases from Rhizopus oryzae [DOI]
- 8 Optimization for cellulase production by Aspergillus niger using saw dust as substrate [DOI]
- 9 Production Of Cellulases By Aspergillus Heteromorphus From Wheat Straw Under Submerged Fermentation [DOI]
- 10 Cellulase Production of Trichoderma reesei Rut C 30 Using Steam-Pretreated Spruce [DOI]
- 11 Production Physiology of Cellulases and β-glucosidase Enzymes of Aspergillus niger Grown under Solid State Fermentation Conditions [DOI]
- 12 Screening, mutagenesis and protoplast fusion of Aspergillus niger for the enhancement of extracellular glucose oxidase production [DOI]
- 13 Fungal Strain Improvement for Cellulase Production Using Repeated and Sequential Mutagenesis [DOI]
- 14 Cellulase Production by Trichoderma longi, Aspergillus niger and Saccharomyces cerevisae Cultured on Waste Materials from Orange [DOI]
- 15 Extra Cellular Endoglucanase Production by Rhizopus oryzae in Solid and Liquid State Fermentation of Agro Wastes [DOI]
- 16 Orange bagasse as substrate for the production of pectinase and laccase by Botryosphaeria rhodina MAMB-05 in submerged and solid state fermentation [DOI]
- 17 A Statistical Approach for Optimization of Simultaneous Production of β -Glucosidase and Endoglucanase byRhizopus oryzaefrom Solid-State Fermentation of Water Hyacinth Using Central Composite Design [DOI]
- 18 Versatile Fluorescent Staining of Fungi in Clinical Specimens by Using the Optical Brightener Blankophor [DOI]
- 19 A hypercellulolytic mutant of Fusarium oxysporum [DOI]
- 20 The General Transcriptional Repressor Tup1 Is Required for Dimorphism and Virulence in a Fungal Plant Pathogen [DOI]
- 21 Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production [DOI]
- 22 MUTATION INDUCED ENHANCED BIOSYNTHESIS OF LIPASES BY RHIZOPUS OLIGOSPORUS VAR. MICROSPORUS
- 23 Chemically treated strain improvement of Aspergillus niger for enhanced production of glucose oxidase.
- 24 Preparation of immobilized cellulase beads and their application to hydrolysis of cellulosic materials [DOI]
- 25 Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes [DOI]
- 26 Effect of Extracellular Factors on Growth and Dimorphism of Rhizopus oryzae with Multiple Enzyme Synthesizing Ability [DOI]
- 27 Enhanced production and characterization of a novel -D-glucose:oxygen-1-oxidoreductase by using Aspergillus niger UV-180-C mutant strain [DOI]
- 28 Enzymes: screening, expression, design and production. [DOI]
- 29 Extracellular β-amylase from Syncephalastrum racemosum [DOI]
Cited by 5
Nagwa. M. Abdel-Aziz, A. Atallah, S. Abd-El-Aal · Arab Universities Journal of Agricultural Sciences · 2019
S. Akbar, R. Prasuna, Rasheeda Khanam · 2015
S. Akbar, R. Prasuna, Rasheeda Khanam · 2013
B. Ghosh, R. Ray · 2013
N. Sharma · 2013
Related research
- Production and Partial Purification of Cellulase from a New Isolate, Penicillium verruculosum BS3 — shares topic coverage
- Cellulase Production by Bacteria: A Review — shares topic coverage
- Isolation and Identification of Cellulolytic and Xylanolytic Bacteria from Huancarhuaz Hot Spring, Peru — shares topic coverage
- Effect of Temperature on Production of Endoglucanase and Xylanase from Some Green Manure Fungal Decomposers — shares topic coverage
- Comparative Production of Cellulase from Chemical and Biological Pretreated Groundnut Husk — shares topic coverage
Article metrics
Real usage data collected on this platform.
57
Page views
0
PDF downloads
24
Outbound clicks
5
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.