Skip to content
Research Article Open access CC BY 4.0

In vivo Toxicity Assessment after the Detoxification of Aflatoxin Compounds – Contaminated Wheat and Corn by Ozone Gas

T. T. El-Sisy, Asmaa A. Salem, Nivin S. Nail, Jehan B. Ali

Annual Research & Review in Biology · pp. 1–18 · Published 23 Jan 2020

10.9734/arrb/2019/v34i330151

Abstract

Aflatoxins (AFs) are dangerous mycotoxins, which include a great number of lipophilic molecules produced by aerobic microscopic fungi belonging to the genus Aspergillus causes health hazard including death to human and livestock. The objective of this study was to evaluate the effectiveness of ozone gas treatment on the fungal growth and detoxification of AFs - contaminated wheat and corn grains. Ozone concentration treatments 40 mg / Kg wheat for 1 hour and 80 mg / Kg corn for 2 hours of exposure time respectively were applied to contaminated samples of wheat and corn grains. It was observed that completely inhibition of Aspergillus growth and consequently the total aflatoxin content was decreased. In vivo, the biosafety assessment for 72 male albino rats fed on diet containing 70% wt. of ozone treated AFs – contaminated grains were evaluated comparing to control groups. Results indicated that rats fed on AFs contaminated grains have significantly increased the serum enzymes activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), malondialdehyde (MDA) content as well as the serum levels of creatinine, urea, glucose, total cholesterol (TC) and triglycerides (TG). Also, it was observed that a significant decrease in the level of serum total protein (TP), albumin (Alb), reduced glutathione (GSH) and testosterone hormone comparing to control groups.  However, the oral administration of ozonized groups ameliorated the biochemical parameters compared to rats fed on contaminated grains. Moreover, histopathological studies of liver, kidney and testis tissues of rats fed on contaminated grains that revealed different lesions and changes in tissues, inversely to that improving effects in tissues of ozonized contaminated grains – fed rats. It was concluded that ozonization treatment were most effective in reduction of mold count and degradation of aflatoxins content for grains during storage.

Aspergillus Aflatoxins ozone gas storage wheat corn biochemical parameters.

References (33)

  1. 1 Theory and Practice of Histological Techniques
  2. 2 Theory and Practice of Histological Techniques [DOI]
  3. 3 Application of Ozone for Enhancing the Microbiological Safety and Quality of Foods: A Review [DOI]
  4. 4 THE AMERICAN ASSOCIATION OF CEREAL CHEMISTS [DOI]
  5. 5 Ozone in the food industry: Principles of ozone treatment, mechanisms of action, and applications: An overview [DOI]
  6. 6 Physicochemical and Functional Properties of Ozone-Oxidized Starch [DOI]
  7. 7 Ozone treatment efficiency on Fusarium graminearum and deoxynivalenol degradation and its effects on whole wheat grains (Triticum aestivum L.) quality and germination [DOI]
  8. 8 Effect of Ozone Treatment on Deoxynivalenol and Wheat Quality [DOI]
  9. 9 Detoxification of aflatoxin in corn flour by ozone [DOI]
  10. 10 Effect of deoxynivalenol detoxification by ozone treatment in wheat grains [DOI]
  11. 11 Degradation of aflatoxins in peanut kernels/flour by gaseous ozonation and mild heat treatment [DOI]
  12. 12 Aflatoxicosis in turkey poults is prevented by treatment of naturally contaminated corn with ozone generated by electrolysis [DOI]
  13. 13 Ozone application in a modified screw conveyor to treat grain for insect pests, fungal contaminants, and mycotoxins [DOI]
  14. 14 Effect of Ozone Gas on Degradation of Aflatoxin B1 and Aspergillus Flavus Fungal [DOI]
  15. 15 Effect of drying and ozonation process on naturally contaminated wheat seeds [DOI]
  16. 16 Effect of ozone gas processing on physical and chemical properties of wheat proteins [DOI]
  17. 17 Detection of serum AFB1-lysine adduct in Malaysia and its association with liver and kidney functions [DOI]
  18. 18 Disinfestation of stored wheat grain infested with Rhyzopertha dominica by ozone treatment: process optimization and impact on grain properties [DOI]
  19. 19 Efficacy of ozone in the microbiological disinfection of maize grains [DOI]
  20. 20 Mycological survey and ochratoxin A natural contamination of swine feedstuffs in Rio de Janeiro State, Brazil [DOI]
  21. 21 Reducing the Histopathological and Biochemical Toxicity of Aflatoxins Contaminated Soybean Using Ozone Treatment [DOI]
  22. 22 Anti-microbial activity [DOI]
  23. 23 Effects of dietary exposure to aflatoxins on some plasma biochemical indices of common carp (Cyprinus carpio) [DOI]
  24. 24 Ozone saturation and decomposition kinetics in porous medium containing different hybrids of maize [DOI]
  25. 25 The effects of low levels of aflatoxin B1 on health, growth performance and reproductivity in male rabbits [DOI]
  26. 26 Ozone treatment effects on microbial count on maize [DOI]
  27. 27 Effect of Ozone-Treated Aflatoxin Contaminated Diets on DNA Damage, Expression of Androgen and Androgen Receptor Genes and Histopathological Changes in Japanese Quail
  28. 28 Biological and Histopathological Evaluations of Using Ozone Gas in Decontamination of AflatoxinB1 in Wheat Grains [DOI]
  29. 29 REMOVAL OF ZEARALENONE FROM WATER BY MEANS OF OZONATION AND INTEGRATED SYSTEM OF OZONATION/NANOFILTRATION USUWANIE ZEARALENONU Z WODY W PROCESIE OZONOWANIA ORAZ W UK£ADZIE OZONOWANIE/NANOFILTRACJA
  30. 30 Effect of Grape Seeds Extract on Attenuation of Aflatoxins Toxicity in Rats [DOI]
  31. 31 Iranian Journal of Fisheries Sciences [DOI]
  32. 32 Journal of Food Processing and Preservation [DOI]
  33. 33 Evaluation of aflatoxin-related products from ozonated corn [DOI]

Cited by 2

In vivo and in vitro toxicity assessment of aflatoxin B1 and its degradation products following a new ozonation method treatment in corn

M. Mahmoudi-Meymand, A. Rahmani, E. Zayerzadeh · World Mycotoxin Journal · 2026

List of Reviewers

· Counselling Psychology Quarterly · 2020

Related research

Article metrics

Real usage data collected on this platform.

63

Page views

0

PDF downloads

26

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.