In Vivo Antitumor Activity of Metal Silver and Silver Nanoparticles in the L5178Y-R Murine Lymphoma Model
José H. Lara-González, Ricardo Gomez-Flores, Patricia Tamez-Guerra, Enriqueta Monreal-Cuevas, Reyes Tamez-Guerra, Cristina Rodríguez-Padilla
Journal of Advances in Medicine and Medical Research · pp. 1308–1316 · Published 12 Apr 2013
10.9734/BJMMR/2013/3108Abstract
Aims: To evaluate the antitumor potential of metal silver and polyvinilpyrrolidone nanoparticle-encapsulated silver on L5178Y-R murine lymphoma cell growth and survival of tumor-bearing mice. Study Design: In vitro and in vivo (pre-clinical) study. Place and Duration of Study: Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, N.L., México, from January 2009 to December 2011. Methodology: Concentration-response cell viability assay was performed in vitro and mice survival studies were done using a L5178Y-R tumor-bearing mouse model. The PROBIT regression analysis was performed to determine the in vitro LC50. In vivo survival distributions were calculated by Kaplan-Meier and Cutler-Ederer analysis, and survival curves comparisons and hypothesis testing was done using the log-rank method. Results: Metal silver induced up to 100% L5178Y-R cells cytotoxicity, with an LC50 of 1.8 X 10-8 M, whereas silver nanoparticles caused up to 78% cytotoxicity, with an LC50 of 14.4 X 10-8 M. In addition, Intramuscular administration of metal silver and silver nanoparticles administered at the time of tumor injection significantly (P = .05) increased mice survival, where 70% and 60% of mice survived at day 35 respectively, as compared with such treatments administered 7 days after tumor induction (55% and 25% survival respectively); vincristine treatment caused 50% mice survival and tumor-bearing control mice had 20% survival. These results open further approaches on treating several types of cancer using free and nanoparticle-encapsulated silver-based therapies.
Cited by 16
Christy Maksoudian, Neshat Saffarzadeh, Evelien Hesemans · Nanoscale Advances · 2020
Kaja Urbańska, Beata Pająk, Arkadiusz Orzechowski · Nanoscale Research Letters · 2015
Mahendra Rai, Avinash P. Ingle, Sonal Birla · Critical Reviews in Microbiology · 2015
Lawrence M. Friedman, Curt D. Furberg, David L. DeMets · Fundamentals of Clinical Trials · 2015
D. Pissuwan · Monitoring and Evaluation of Biomaterials and their Performance In Vivo · 2017
Kaushik Banerjee, Satyajit Das, Pritha Choudhury · Chemotherapy · 2017
R. Sakthi Devi, Agnishwar Girigoswami, M. Siddharth · Applied Biochemistry and Biotechnology · 2022
Inès Karmous, Ashish Pandey, Khemais Ben Haj · Biological Trace Element Research · 2019
Reena Singh, Sunil Kumar Sahu, Muthusamy Thangaraj · Journal of Nanoparticles · 2014
Shichang Yu, Liang Zhao, Weiya Kang · Inorganic Chemistry Communications · 2024
Related research
- A Rare Case of Kikuchi-Fujimoto Disease in an Indian Female — shares topic coverage
- Association of Hepatitis Virus Infections with CD4 and CD8 among Certain Group of Lymphoma Patients in Babylon Provence — shares topic coverage
- Feline Mesenteric form of Multinodal Lymphoma: A Case Report — shares topic coverage
- Thoracoscopy as the Method of Choice in Diagnosis of Mediastinal Lymphadenopathy — shares topic coverage
- An Immunohistochemical Study of PRAME Expression in Non-hodgkin’s Lymphoma — shares topic coverage
Article metrics
Real usage data collected on this platform.
1
Page views
0
PDF downloads
0
Outbound clicks
16
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.