In vitro Study of Neem Bark Extract on Rumen Fermentation and Biohydrogenation of Polyunsaturated Fatty Acids
Asian Journal of Research in Animal and Veterinary Sciences · pp. 37–47 · Published 26 Feb 2024
10.9734/ajravs/2024/v7i1284Abstract
Aim: An In vitro gas production technique was conducted to assess effects of commercial Neem (Azadirachta indica) bark extract (NBE) on rumen fermentation and biohydrogenation (BH) of selected polyunsaturated fatty acids (PUFAs). Study Design: There were three (3) treatments, each made up of a total of 32 incubation bottles i.e. 8 bottles for control (contained basal diet, rumen fluid and buffer solution), 8 bottles (basal diet, rumen fluid, buffer solution and 100 mg/l of NBE), 8 bottles (basal diet, rumen fluid, buffer solution and 200 mg/l of NBE) and 8 bottles (basal diet, rumen fluid, buffer solution and 400 mg/l of NBE). Methodology: All three (3) treatments were incubated at a temperature of 39oC for 12 hours (treatment 1), 24 hours (treatment 2) and 48 hours (treatment 3). During incubation procedure, gas production was measured by using pressure transducer after 3, 6, 9, 12, 24, and 36 hours. Consequently, pH was measured by using pH meter after 12, 24 and 48 hours of fermentation and then incubation was stopped. During this time, digested samples in gas medium were collected for analysis of PUFAs by using gas chromatography. GenStat 15th edition (version 15.1) software was used to analyse collected data in which statistical method of analysis of variance (ANOVA) i.e. one – way ANOVA was used to compare means between treatments. Results: Results showed that no differences in gas production, pH, changes in concentration and BH of selected PUFAs after 48 hours of in vitro fermentation. However, concentrations (g/100g FA) of linolenic acid at levels of 200 and 400 mg/l of NBE i.e. 0.8 and 0.8 respectively were increased (p< .05) compared to control (0.7). Moreover, NBE at level of 400 mg/l inhibits (p<.05) BH of linolenic acid (92.1%) and docosahexaenoic acid (75.0%) compared to control (92.8%) and (80.3%) respectively. Conclusion: These findings demonstrated that, NBE possesses bioactive compounds which affect BH of docosahexaenoic and linolenic acids and has little effect on improving fermentation and modifying ruminal BH of PUFAs.
References (47)
- 1 A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds [DOI]
- 2 Lipid Metabolism in the Rumen [DOI]
- 3 Invited Review: Essential Oils as Modifiers of Rumen Microbial Fermentation [DOI]
- 4 Fatty acid composition, including conjugated linoleic acid, of intramuscular fat from steers offered grazed grass, grass silage, or concentrate-based diets. [DOI]
- 5 A review of plant-derived essential oils in ruminant nutrition and production [DOI]
- 6 A semi-automated in vitro gas production technique for ruminant feedstuff evaluation [DOI]
- 7 Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle [DOI]
- 8 Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds [DOI]
- 9 Ruminal pH regulation and nutritional consequences of low pH [DOI]
- 10 Lipid metabolism in the rumen: New insights on lipolysis and biohydrogenation with an emphasis on the role of endogenous plant factors [DOI]
- 11 Effect of Essential Oil Active Compounds on Rumen Microbial Fermentation and Nutrient Flow in In Vitro Systems [DOI]
- 12 Dietary sources of unsaturated fatty acids for animals and their transfer into meat, milk and eggs: A review [DOI]
- 13 Effect of plant extracts on in vitro methanogenesis, enzyme activities and fermentation of feed in rumen liquor of buffalo [DOI]
- 14 Modelling the implications of feeding strategy on rumen fermentation and functioning of the rumen wall [DOI]
- 15 Effects of condensed tannins from Leucaena on methane production, rumen fermentation and populations of methanogens and protozoa in vitro [DOI]
- 16 Effects of Essential Oils on Rumen Fermentation, Microbial Ecology and Ruminant Production [DOI]
- 17 DHA content in milk and biohydrogenation pathway in rumen: a review [DOI]
- 18 Rumen biohydrogenation of n -3 polyunsaturated fatty acids and their effects on microbial efficiency and nutrient digestibility in sheep [DOI]
- 19 Clinical Examination of Farm Animals [DOI]
- 20 Biohydrogenation of dietary n -3 PUFA and stability of ingested vitamin E in the rumen, and their effects on microbial activity in sheep [DOI]
- 21 REVIEW ON NEEM (AZADIRACHTA INDICA): THOUSAND PROBLEMS ONE SOLUTION
- 22 Effect of Azadirachta indica on paracetamol-induced hepatic damage in albino rats [DOI]
- 23 Diurnal Variation in Ruminal pH on the Digestibility of Highly Digestible Perennial Ryegrass During Continuous Culture Fermentation [DOI]
- 24 Effects of feeding flaxseed or sunflower-seed in high-forage diets on beef production, quality and fatty acid composition [DOI]
- 25 Effect of Supplementation with Calcium Salts of Fish Oil on n-3 Fatty Acids in Milk Fat [DOI]
- 26 The role of pH on the survival of rumen protozoa in steers [DOI]
- 27 Effects of sunflower oil infusions of Asparagopsis taxiformis on in vitro ruminal methane production and biohydrogenation of polyunsaturated fatty acids [DOI]
- 28 Effect of feeding water washed neem (Azadirachta indica) seed kernel cake on the quality, lipid profile and fatty acid composition of goat meat [DOI]
- 29 Perioperative ruminal pH changes in domestic sheep (Ovis aries) housed in a biomedical research setting.
- 30 The effects of bovine milk fat on human health [DOI]
- 31 Effects of chemically or technologically treated linseed products and docosahexaenoic acid addition to linseed oil on biohydrogenation of C18:3n-3 in vitro [DOI]
- 32 Characterization of the disappearance and formation of biohydrogenation intermediates during incubations of linoleic acid with rumen fluid in vitro1 [DOI]
- 33 Efficiency of ethanolic extract of peppermint (Mentha piperita) as an antibiotic growth promoter substitution on performance, and carcass characteristics in broiler chickens [DOI]
- 34 Chrysanthemum coronarium as a modulator of fatty acid biohydrogenation in the rumen [DOI]
- 35 The polyunsaturated fatty acid composition of beef and lamb liver [DOI]
- 36 In vitro biohydrogenation of four dietary fats [DOI]
- 37 Chemical and nutritional evaluation of neem oil [DOI]
- 38 Temperature and duration of heating of sunflower oil affect ruminal biohydrogenation of linoleic acid in vitro [DOI]
- 39 Neem oil modulates rumen fermentation properties in a continuous cultures system [DOI]
- 40 In vitro rumen biohydrogenation of unsaturated fatty acids in tropical grass-legume rations [DOI]
- 41 Changes of hemoglobin content and glucose levels in the blood of Rattus norvegicus by water extracts of Azadirachta indica [DOI]
- 42 Effects of dietary fibre level on rumen pH, total microbial count and methanogenic archaea in Bonsmara and Nguni steers [DOI]
- 43 Degradation of ochratoxin a in rumen fluid in vitro
- 44 Effect of water-washed neem (Azadirachta indica) fruit on rumen digesta fatty acids and biohydrogenation intermediates of fattened West African dwarf rams [DOI]
- 45 Chevon characteristics of goats fed diets with water washed neem (Azadirachta indica) seed kernel cake [DOI]
- 46 African Journal of Agricultural Research [DOI]
- 47 International Research Journal Of Pharmacy [DOI]
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