In vitro Study Anti-proliferative Potential of Algae Extract against Cancer Cell Line

Abstract

Background: Oxidative stress is defined as imbalance between oxidant and antioxidant ratio, that lead to oxidative damage of biologically active molecules, finally lead to different disease and initiate carcinogenesis. The drug discovery using natural products as medicinal plants or marine organism still an important target for recent research. This study investigated anticancer activity of algae extract obtained from Red sea at Jeddah. Materials and Methods: Aqueousand methanol extracts ofDictyotaciliolata(DC) were tested on HCT-116 and HepG2 cell lines using WST-1. Aqueous extract (AEDC) and methanol extract (MEDC) at doses 0.05, 0.1, 0.5, 1 mg/ml and positive control 0.3% H2O2 at doses 0.5 mg/ml for 24,48 and 72 h (for two cell lines). Results: AEDC showed the mosteffective antitumor activity against HCT116 and HePG2, the IC50 dose for HCT116 cells was 0.05 mg/ml at 72 h, while for the HePG2 it was 0.01 mg/ml at 72 h. These results showed that HePG2cells was more sensitive to the AEDC. However, IC50 for MEDC were 0.01 mg/ml for HCT116 and 0.05 for HepG2 at 48 Hrs. The algae extracts contain sulfated polysaccharides and different pigments as chlorophylls, carotenoids and phycobiliproteins. These pigments were approved as biological active biomolecules that exert different biological activities as antioxidants, antitumor and rich source of micronutrients. In addition, AEDC or MEDC exert apoptotic activity by increase activity of caspase 3 and 9 in HepG2. Conclusıon: The antitumor effect of AEDC or MEDC is promising for development of chemotherapeutic agent as effective with no side effects.