In vitro Screening of Some Indian Medicinal Plants for Their Activity against Extended Spectrum Beta Lactamases
Anupma Malik, Sheema Bai, Rahul Taneja, Sunita Dalal
Biotechnology Journal International · pp. 1–10 · Published 30 Jun 2015
10.9734/BBJ/2015/18406Abstract
Context: Extended Spectrum Beta Lactamases (ESBLs) play an important role in pathogenesis of various infections by enabling the bacterial species to be resistant to β-lactam antibiotics including extended-spectrum cephalosporins. Plants were selected on the basis of their traditional applications. Objective: Our investigation screens and evaluates15 Indian medicinal plants for antimicrobial efficacy and synergistic potential against ESBLs producing bacteria. Materials and Methods: 6 bacterial strains were screened for their ability to release ESBLs. Plant extracts in methanol and aqueous solvents were screened for their effect on ESBLs producing bacteria. Antimicrobial-linked ESBLs inhibition activity and minimum inhibitory concentration (MIC) of extracts were evaluated by agar well diffusion and microdilution method. Synergistic interactions between plant extracts exhibiting good antimicrobial activity and extended spectrum cephalosporins were explored by Checkerboard method. Results: Three strains were detected as ESBL positive. The results of susceptibility assay clearly showed strong ESBLs inhibitory effect of Crinum latifolium, Oroxylem indicum, Punica granatum, Sapindus emarginatus and Terminalia chebula and their MIC values ranged from 1.87-30 mg/ml. In vitro interactions between plant extracts and antibiotics cefotaxime and ceftaizidime evaluated in terms of fractional inhibitory concentration (FIC) indices indicated synergism. Discussion and Conclusion: Higher resistance of ESBLs positive strains to β-lactam antibiotics encourages us to search the novel ESBLs inhibitors. Maximum 10-fold decline in the MIC of antibiotics cefotaxime and ceftaizidime was achieved in combination with plant extracts. Owing to enormous clinical significance of ESBL-producing organisms coupled with limited therapeutic options, the results revealed by present study are of paramount importance.
References (15)
- 1 Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically
- 2 Extended-Spectrum β-Lactamases: a Clinical Update [DOI]
- 3 Extended-Spectrum β-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat [DOI]
- 4 Growing Group of Extended-Spectrum β-Lactamases: the CTX-M Enzymes [DOI]
- 5 Experiments in Microbiology, Plant Pathology and Biotechnology
- 6 In vitro antibacterial activities of tigecycline in combination with other antimicrobial agents determined by chequerboard and time-kill kinetic analysis [DOI]
- 7 In vitro efficacy of bioactive extracts of 15 medicinal plants against ESβL-producing multidrug-resistant enteric bacteria [DOI]
- 8 Presence of different beta-lactamase classes among clinical isolates of Pseudomonas aeruginosa expressing AmpC beta-lactamase enzyme [DOI]
- 9 TRANSFERABLE ENZYMATIC RESISTANCE TO THIRD-GENERATION CEPHALOSPORINS DURING NOSOCOMIAL OUTBREAK OF MULTIRESISTANT KLEBSIELLA PNEUMONIAE [DOI]
- 10 High Levels of Antimicrobial Coresistance among Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae [DOI]
- 11 Antibacterial activity of Rosmarinus officinalis L. alone and in combination with cefuroxime against methicillin–resistant Staphylococcus aureus [DOI]
- 12 Antibacterial Activity of the Extracts Obtained from Rosmarinus officinalis, Origanum majorana, and Trigonella foenum-graecum on Highly Drug-Resistant Gram Negative Bacilli [DOI]
- 13 Inhibition of Beta-Lactamase by 1,4-Naphthalenedione from the Plant Holoptelea integrifolia [DOI]
- 14 Synergistic Killing Effect of Synthetic Peptide P20 and Cefotaxime on Methicillin-Resistant Nosocomial Isolates of Staphylococcus aureus
- 15 Antibacterial activity of bark extracts of Terminalia arjuna (Roxb .) against Extended Spectrum -Lactamase producing multi drug resistant Bacteria from Urinary Tract Infections
Cited by 9
R. Torres-Martínez, A. Moreno-León, Y.M. García-Rodríguez · Letters in Applied Microbiology · 2022
Yazan Ismail, Mohammed Wedyan, Muad Al-zu`abe · Research Journal of Medicinal Plants · 2016
Mohammad Jalal, Mohammad Azam Ansari, Syed Ghazanfar Ali · Artificial Cells, Nanomedicine, and Biotechnology · 2018
Showing 3 of 9 known citations — external sources report more than can currently be individually listed.
Related research
- Antibacterial Activities of Streptomyces collinus and Streptomyces refuineus from Cocoa, Orange and Timber Plantation Soils in Akure Metropolis — shares topic coverage
- Antimicrobial Activity, Phytochemical Screening and Nutrient Analysis of Tetrapleura tetraptera and Piper guineense — shares topic coverage
- Antibacterial and Antibiofilm Properties of the Crude Ethanolic, Methanolic and Aqueous Bark and Seed Extracts of Citrullus lanatus Fruit — shares topic coverage
- Susceptibility of Some Clinically Resistant Bacterial Isolates to Thyme Essential Oil, Chitosan and Lactobacillus reuteri — shares topic coverage
- Antibacterial and Antifungal Effect of Cinnamon — shares topic coverage
Article metrics
Real usage data collected on this platform.
49
Page views
0
PDF downloads
23
Outbound clicks
9
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.