Skip to content
Research Article Open access CC BY 3.0

In vitro Grafting of Selected Papaya (Carica Papaya L.) Lines in Kenya

Naomi Nzilani Mumo, Fredah Karambu Rimberia, George Edward Mamati, Agnes Wanjiru Kihurani

Annual Research & Review in Biology · pp. 2337–2346 · Published 5 Apr 2014

10.9734/ARRB/2014/9494

Abstract

The aim of this study was to evaluate success of In vitro grafting method in three selected Kenyan papaya lines. In vitro regenerated shoots of about 20mm were used where the upper 10mm tips were excised and used as scions while the remaining portion was used as rootstock. The rootstocks and the scions were used interchangeably. Number of leaves and length of grafts were recorded every week for six weeks. Number of scions that were alive after six weeks was also recorded. Grafted shoots were rooted on MS with 2.5mg/l IBA. The number of grafted shoots that were alive after six weeks ranged between 45% and 80%. The types of rootstock and scions affected the number of leaves and length of grafted shoots with lines 1 and line 2 grafted on their rootstock exhibiting the highest leaf number and shoot length. Papaya line 3 grafted on either line 1 or 2 rootstock and vice versa had a higher number of leaves and shoot length as compared with line 3 grafted on its own rootstock. Within 24 weeks, in vitro grafted plantlets were achieved. In vitro grafting of selected papaya lines was successful.

Carica papaya In vitro grafting lines propagation success

References (25)

  1. 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
  2. 2 Somatic Embryogenesis: A Model for Early Development in Higher Plants. [DOI]
  3. 3 Anatomy and physiology of graft incompatibility in solanaceous plants [DOI]
  4. 4 Improvement of Shoot-tip Grafting in vitro for Virus-free Citrus1 [DOI]
  5. 5 Revisiting the enzymes stored in the laticifers of Carica papaya in the context of their possible participation in the plant defence mechanism [DOI]
  6. 6 A MODEL FOR GRAFT COMPATIBILITY‐INCOMPATIBILITY IN HIGHER PLANTS [DOI]
  7. 7 STUDIES OF VEGETATIVE COMPATIBILITY‐INCOMPATIBILITY IN HIGHER PLANTS. I. A STRUCTURAL STUDY OF A COMPATIBLE AUTOGRAFT IN SEDUM TELEPHOIDES (CRASSULACEAE) [DOI]
  8. 8 In vitro and in vivo germination of papaya (Carica papaya L.) seeds [DOI]
  9. 9 Micrografting and its Applications to Tree Improvement [DOI]
  10. 10 Water Transfer at Graft Union of Tomato Plants Grafted onto Solanum Rootstocks [DOI]
  11. 11 A Study of the graft union in in vitro micrografted apple [DOI]
  12. 12 Morphological diversity of Kenyan papaya germplasm
  13. 13 IN VITRO PRODUCTION OF VIRUS-FREE PEAR PLANTS [DOI]
  14. 14 In vitro regeneration of selected Kenyan papaya ( Carica papaya L. ) lines through shoot tip culture [DOI]
  15. 15 Some chemical characteristics and availability of vitamin A and vitamin C from Kenyan varieties of papayas (Carica papaya L.) [DOI]
  16. 16 Breeding Papaya (Carica papaya L.) [DOI]
  17. 17 GRAFTING PAPAYAS (CARICA PAPAYA L.) [DOI]
  18. 18 In vitro micro-grafting of some Iranian grapevine cultivars.
  19. 19 AN IMPROVED TECHNIQUE OF PROPAGATING 'EKSOTIKA' PAPAYA [DOI]
  20. 20 IN VITRO ROOTING OF PORTUGUESE PEAR CULTIVARS (PYRUS COMMUNIS) IN RESPONSE TO CHANGES IN AUXIN INDUCTION AND DARK PERIOD TREATMENTS [DOI]
  21. 21 INCOMPATIBILITY IN CULTURED EXPLANT‐GRAFTS BETWEEN MEMBERS OF THE SOLANACEAE [DOI]
  22. 22 In-vitro plantlet regeneration from shoot tip of field-grown hermaphrodite papaya (Carica papaya L. cv. Eksotika)
  23. 23 Somatic Embryogenesis in Papaya (Carica papaya L.) [DOI]
  24. 24 EVALUATION OF GRAFT-TAKE USING A THERMAL CAMERA [DOI]
  25. 25 Plant Propagation: Principles and Practices [DOI]

Cited by 3

Optimized protocol for high-frequency papaya propagation: morpho-stereomicroscopic analysis and genetic fidelity assessment

Vishal, Gurupkar Singh Sidhu, Popat Nanaso Gaikwad · Plant Cell, Tissue and Organ Culture (PCTOC) · 2024

Showing 1 of 3 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

63

Page views

0

PDF downloads

10

Outbound clicks

3

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.