In vitro Detoxification of Aflatoxin B1 by Calcium Bentonite Clay Supplementation in Aflatoxigenic Mould Contaminated Feeds for Nile Tilapia, Oreochromis niloticus (Linnaeus, 1758)
Remisha Olokkaran, Saleena Mathew
Asian Journal of Fisheries and Aquatic Research · pp. 1–18 · Published 13 Nov 2020
10.9734/ajfar/2020/v10i130169Abstract
Aflatoxins are toxic, carcinogenic secondary metabolites mainly produced by Aspergillus flavus fungi that is often detected in food and agricultural commodities. Calcium bentonite clay has been used historically as an anti-caking agent and a feed binder and demonstrated the ability to bind aflatoxins. Feed samples were prepared for Nile tilapia to determineaflatoxin detoxification properties of calcium bentonite clay. 0.5%, 1%, 1.5% and 2% of this clay was incorporated into Aspergillus flavus contaminated feed and labeled as TF1, TF2, TF3, TF4, and TF5 respectively. The determination and quantification of aflatoxins produced by the fungus in the feed samples were carried out by HPTLC method. The RF values of all the feed samples were confirmed to be the presence of aflatoxin B1 and AFB2, G1 and G2 toxins were not found in any of the samples. Results from this study revealed that the calcium bentonite clay at 2% effectively reduced AFB1 toxin by its adsorption properties. Moreover, calcium bentonite clay improved water stability, water absorption rate with minimal disintegration and nutrients leaching of feed samples in water.
References (20)
- 1 Compendium of Soil Fungi [DOI]
- 2 Global overview on the use of fish meal and fish oil in industrially compounded aquafeeds: Trends and future prospects [DOI]
- 3 Dietary clay in the chemoprevention of aflatoxin-induced disease [DOI]
- 4 A review of the effects of ingredient composition and processing conditions on the physical qualities of extruded high-energy fish feed as measured by prevailing methods [DOI]
- 5 Aflatoxins in aquatic species: metabolism, toxicity and perspectives [DOI]
- 6 Aflatoxin Contamination in Food and Body Fluids in Relation to Malnutrition and Cancer Status in Cameroon [DOI]
- 7 Physiological and toxicological responses in rats fed aflatoxin-contaminated diet with or without sorbent materials [DOI]
- 8 Prevention of aflatoxicosis by addition of hydrated sodium calcium aluminosilicate to the diets of growing barrows [DOI]
- 9 Protective Effects of Sodium Selenite against Aflatoxin B1-Induced Oxidative Stress and Apoptosis in Broiler Spleen [DOI]
- 10 Adsorption of aflatoxin B1 on montmorillonite [DOI]
- 11 The Effect of Aflatoxin-B1 on Red Drum (Sciaenops ocellatus) and Assessment of Dietary Supplementation of NovaSil for the Prevention of Aflatoxicosis [DOI]
- 12 Aflatoxin levels in common foods of South Western Uganda: a risk factor to hepatocellular carcinoma [DOI]
- 13 Impact of mechanical shelling and dehulling onFusariuminfection and fumonisin contamination in maize [DOI]
- 14 The role of bentonite particle size distribution on kinetic of cation exchange capacity [DOI]
- 15 Composition, preparation and utilization of feeds for Crustacea [DOI]
- 16 Effects of dietary protein concentration on growth and muscle composition of juvenile Zacco barbata [DOI]
- 17 Reduction of the bioavailability of 20 μg/kg aflatoxin in trout feed containing clay [DOI]
- 18 Multiple Metastasis of Rainbow Trout Hepatoma [DOI]
- 19 Effect of Moisture Content, Processing Water Temperature, and Immersion Time on Water Stability of Pelleted Shrimp Diets [DOI]
- 20 Bentonite Reduces Measurable Aflatoxin B1in Fish Feed [DOI]
Cited by 2
Darci Carlos Fornari, Sílvio Peixoto, Steven P. Ksepka · Frontiers in Animal Science · 2023
Showing 1 of 2 known citations — external sources report more than can currently be individually listed.
Related research
- The Effect of Aspergillus flavus ES Isolate on the Decolourisation of Crystal Violet, Titan Yellow and Congo Red — shares topic coverage
- Differential Expression Pattern of Heat Shock Protein Genes in Toxigenic and Atoxigenic Isolate of Aspergillus flavus — shares topic coverage
- Enhancement Biochemicals Levels and Improve Histological Tissues of Kidney and Liver Organs for Duckling’s Received Aflatoxin B1 by Using Propolis Extract — shares topic coverage
- In vivo Efficacy of Posaconazole (POS) against Voriconazole Resistant (VCZ-R) Aspergillus flavus in an Inhalational Neutropenic Murine Model of Invasive Pulmonary Aspergillosis — shares topic coverage
- Sub Acute Toxicity Study of Mixed Fungal Culture Filtrates Isolated from Maize Stalks of Shivamogga District in Rats — shares topic coverage
Article metrics
Real usage data collected on this platform.
2
Page views
0
PDF downloads
0
Outbound clicks
2
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.