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Research Article Open access CC BY 4.0

In-vitro Cytotoxicity of Extracts of Selected Malaria Medicinal Plants Used by Traditional Healers of Kericho East Sub-county, Kenya

Pacifica Chepchumba Bwogo, Rael J. Masai

Journal of Applied Life Sciences International · pp. 96–103 · Published 6 Nov 2023

10.9734/jalsi/2023/v26i5623

Abstract

Background: Malaria is a fatal disease which affects people of all ages; especially pregnant women, young children <5 years, and the elderly because of their weakened immune systems. The currently used anti-malarial drugs have been linked to a variety of negative side effects including the parasite resistance. Additionally, the costs associated with the conventional malaria management approach are arguably high, particularly for people living in low-income countries, highlighting the need for alternative and complementary approaches. Medicinal plants therefore are a viable alternative since they are arguably less expensive and easily accessible. However, there is limited information on safety and efficacy of the plants. This study was designed to investigate the cytotoxic activities of polar and non-polar crude extracts solvents of selected plants used by traditional healers in Kericho East Sub-County to treat malaria. Materials and Methods: Plants studied included Pittosporum viridiflorum (stem barks), Phytolacca dodecandra (Leaves), and Gardenia ternifolia (roots barks). Plant parts selected were collected from Kericho East Sub-county; Kapsoit, Kaitui, and Fort-Ternan. Their crude extracts were obtained from hexane, dichloromethane (DCM), Methanol (MeOH), and 5% H2O/MeOH. In vitro cytotoxic effects and safety of the studied plants' extracts were identified using mammalian Vero E6 cells.  Results: Most of the plants tested yielded impressive cytotoxicity results, indicating that therapeutic doses could be achieved at safe concentrations. However, P. viridiflorum hexane, DCM, MeOH, and 5% H2O/MeOH crude extracts were toxic to the cultured cells expressing the mean CC50 ± SE of 65.11±0.40, 25.63±0.23, 87.94 ±0.59 and 98.54±0.66 μg/ml, respectively. Conclusion: G. ternifolia and P. dodecandra have offered hope in the treatment of malaria since their crude extracts have demonstrated no toxicity. The study found P. viridiflorum crude extracts to be toxic but there is the possibility of isolating safe nontoxic compound/s because they were less toxic at lower doses. This study therefore identified potential plants that could be used to develop novel anti-plasmodial agents.

Anti-malarial drugs anopheles mosquito medicinal plants malaria cytotoxicity

References (22)

  1. 1 Orexin activation counteracts decreases in nonexercise activity thermogenesis (NEAT) caused by high-fat diet [DOI]
  2. 2 Soil-landscape modelling and spatial prediction of soil attributes [DOI]
  3. 3 In vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa [DOI]
  4. 4 Ethnobotanical study of medicinal plants in the Blue Nile State, South-eastern Sudan [DOI]
  5. 5 An Ethnobotanical Survey of Herbal Markets and Medicinal Plants in Lagos State of Nigeria
  6. 6 Potent antimalarial activity of the alkaloid nitidine, isolated from a Kenyan herbal remedy [DOI]
  7. 7 Brine shrimp toxicity and antiplasmodial activity of five Kenyan medicinal plants [DOI]
  8. 8 Antimalarial activity of some plants traditionally used in Meru district of Kenya [DOI]
  9. 9 In vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa [DOI]
  10. 10 Artemisinin resistance in Plasmodium falciparum: what is it really? [DOI]
  11. 11 Anti-malarial activity of withania somnifera L. Dunal extracts in mice.
  12. 12 Antiplasmodial and Cytotoxic Activities of Extracts of Selected Medicinal Plants Used to Treat Malaria in Embu County, Kenya [DOI]
  13. 13 Toxicity and related mechanisms of dihydroartemisinin on porcine oocyte maturation in vitro [DOI]
  14. 14 Testing Beyond Ethnomedical Claims: Brine Shrimp Lethality of Some Tanzanian Plants [DOI]
  15. 15 Antimalarial drugs and the prevalence of mental and neurological manifestations: A systematic review and meta-analysis [DOI]
  16. 16 In vitro Cytotoxic Activity of Aqueous Root Extract of Althea kurdica against Endothelial Human Bone Marrow Cells (line k562) and Human Lymphocytes
  17. 17 Screening of Tropical Medicinal Plants for Molluscicidal, Larvicidal, Fungicidal and Cytotoxic Activities and Brine Shrimp Toxicity [DOI]
  18. 18 Antiplasmodial Activity of the Crude Extract and Solvent Fractions of Stem Barks of Gardenia ternifolia in Plasmodium berghei-Infected Mice [DOI]
  19. 19 Antimalarial drugs and the prevalence of mental and neurological manifestations: A systematic review and meta-analysis [DOI]
  20. 20 Ethanol Extracts from Selected Tanzanian Medicinal Plants Selectively Inhibit Plasmodium falciparum Growth In Vitro [DOI]
  21. 21 In vitro anti-plasmodial activity of crude extracts of Gardenia ternifolia, Pittosporum viridiflorum and Phytolaca dodecandra used for treatment of malaria in Kericho County, Kenya. [DOI]
  22. 22 Evidence-Based Complementary and Alternative Medicine [DOI]

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