In vitro Anticancer and Antioxidative Potential of Nyctanthes arbor-tristis Linn Aqueous Leaves Extract

Abstract

Nyctanthes arbor-tristis is a medicinal plant used to treat various inflammation-associated ailments, cancer and to combat innumerable infections in the traditional system of medicine. The current study’s goal was to investigate the antioxidative and anticancer properties of the aqueous leaves extract of Nyctanthes arbor-tristis (NAE). The leaves of Nyctanthes arbor-tristis were collected from CSIR- CIMAP Research Centre, Pantnagar, Uttarakhand. To examine the antioxidant activity of NAE two different approaches were followed. The aqueous leaves extract showed marked antioxidant potential determined by total antioxidant capacity and reducing power. NAE showed a notable total antioxidant capacity of 235.67 mg of ascorbic acid equivalents per g of extract. The reducing power increased with the increment in the concentration of NAE, ranging from 0.063 at 20μg/mL to 0.388 at 200μg/mL. MTT assay was investigated against HepG2 cell line. High level of resistance to HepG2 liver cancer cell lines was noted with NAE with MNCD value of 10μg/mL. The results obtained highlighted the importance of extracts from Nyctanthes arbor-tristis and suggested the use of chemotherapeutic drugs to treat tumours in the future.