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Research Article Open access CC BY 4.0

In vitro and In vivo Effect of Quercetin on Hepatocyte Transmembrane N+, K+- ATPase Activity in Rats

Akinwumi Tosin Ogundajo, Joshua Oloruntobi Imoru

International Journal of Biochemistry Research & Review · pp. 13–19 · Published 18 Mar 2015

10.9734/IJBCRR/2015/16387

Abstract

Aims: This study investigated In vitro and In vivo effect of quercetin on transmembrane hepatocyte Na+, K+- ATPase activity in normal Wistar rats. Study Design: One factor experimental design. Place and Duration of Study: Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria, between April and September 2010. Methodology: In vitro effect of quercetin on transmembrane hepatocyte Na+, K+-ATPase activity was investigated through enzyme assay by measuring the released inorganic phosphate (Pi) through the method of Fiske and Subbarow, while in In vivo experiment, three (3) main groups of male Wistar rats weighing 200-250 g (n=5) were randomly selected. Group 1 (IP; Intraperitoneal), group 2 (IV; Intravenous) and 3 (OR; Oral) group. All three groups were administered with quercetin 50 mg/kg body weight (once/day) for 5 days through respective routes. The control for each group received equal volume of vehicle through respective routes of administration. Hepatocyte Na+, K+-ATPase activity for each group was assayed after days of quercetin administration. Results: In vitro results indicated that quercetin, with IC50 of 5.75 µm showed high inhibitory activity on Na+, K+-ATPase, while in vivo result showed inhibitory activity on hepatic Na+ K+-ATPase irrespective of the routes of administration. Quercetin through the oral route showed significant    (F5, 12= 265.8; P=0.05) inhibitory effect on hepatic Na+ K+-ATPase compared to control. Administration of quercetin though the parenteral routes (intraperitoneal and intravenous) showed significant (F5, 12= 265.8; P<0.001) inhibitory effect compared to respective control. The parenteral routes of administration of quercetin have significant (F5, 12= 265.8; P<0.001) inhibitory effect on hepatic Na+ K+-ATPase compared to the oral route Conclusion: Quercetin showed high inhibitory effect In vitro on hepatocyte Na+, K+- ATPase activity in normal rats. In vivo administration of quercetin showed significant inhibitory effect on hepatocyte Na+, K+- ATPase activity in normal rats, with the parenteral routes of administration exerting significant inhibitory effect compared to the oral route.

Quercetin hepatocyte Na K -ATPase In vitro In vivo

References (23)

  1. 1 PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT [DOI]
  2. 2 THE COLORIMETRIC DETERMINATION OF PHOSPHORUS [DOI]
  3. 3 Biochemistry of Na,K-ATPase [DOI]
  4. 4 A critical review of the data related to the safety of quercetin and lack of evidence of in vivo toxicity, including lack of genotoxic/carcinogenic properties [DOI]
  5. 5 Na+,K(+)-ATPase. [DOI]
  6. 6 A review of the genetic effects of naturally occurring flavonoids, anthraquinones and related compounds [DOI]
  7. 7 Reduced Progression of Atherosclerosis in Apolipoprotein E–Deficient Mice Following Consumption of Red Wine, or Its Polyphenols Quercetin or Catechin, Is Associated With Reduced Susceptibility of LDL to Oxidation and Aggregation [DOI]
  8. 8 The highly conserved cardiac glycoside binding site of Na,K-ATPase plays a role in blood pressure regulation [DOI]
  9. 9 Quercetin protects against oxidative stress-related renal dysfunction by cadmium in rats [DOI]
  10. 10 Quercetin modulates Na(+)/K(+) ATPase and sodium hydrogen exchanger in type 2 diabetic erythrocytes.
  11. 11 On the mechanism of ionic regulation of apoptosis: would the Na+/K+‐ATPase please stand up? [DOI]
  12. 12 Comparative Studies on Dicholesteroyl Diselenide and Diphenyl Diselenide as Antioxidant Agents and their Effect on the Activities of Na+/K+ ATPase and δ-Aminolevulinic acid Dehydratase in the Rat Brain [DOI]
  13. 13 [43] Role of flavonoids and iron chelation in antioxidant action [DOI]
  14. 14 Structure-function relationships of cation binding in the Na+/K+-ATPase [DOI]
  15. 15 Attachment of rhamnosyl glucoside on quercetin confers potent iron-chelating ability on its antioxidant properties [DOI]
  16. 16 The Effects of Different Antioxidants on the Activity of Cerebrocortical MnSOD and Na,K-ATPase from post mortem Alzheimer’s Disease and Age-matched Normal Brains [DOI]
  17. 17 Role of the liver in the metabolic control of eating: What we know—and what we do not know [DOI]
  18. 18 Metabolic control of eating.
  19. 19 Hemin/nitrite/H2O2 induces brain homogenate oxidation and nitration: effects of some flavonoids [DOI]
  20. 20 The Effect of Galactose Metabolic Disorders on Rat Brain Na+,K+-ATPase Activity [DOI]
  21. 21 Does Na+-K+-atpase have any role in bile secretion? [DOI]
  22. 22 Quercetin Potentiates Hepatoprotective and Antioxidant Response to Intraperitoneal, Intravenous, Subcutaneous and Oral Administration in Wistar Rats [DOI]
  23. 23 The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals [DOI]

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