In vitro and In vivo Antitrypanosomal Effects of Petroleum Ether, Chloroform and Methanol Extracts of Artemisia maritima Linn

Abstract

Petroleum ether, chloroform and methanol extracts of the whole plant of Artemisia maritima Linn were studied in vitro and in vivo for antitrypanosomal activity against Trypanosoma brucei brucei in Swiss albino mice. The extracts were also screened for phytochemicals/secondary metabolites. All the extracts showed trypanocidal activity against T. brucei brucei in vitro with the petroleum ether extract showing the highest activity. The in vivo study revealed that only the chloroform extract A. maritima exhibited antitrypanosomal activity. This extract at a dose of 100mg/kg body weight significantly (p<0.05) reduced the parasitemia in T. brucei brucei infected mice when compared with the other treatment groups. The chloroform extract of A. maritima at this dose reduced the level of parasitemia to 26%. This reduction in the level of parasitemia is statistically significant (p<0.05) compared to the other treatment groups and the untreated control group. The result of the phytochemical analysis revealed that the extracts contain secondary metabolites like flavonoids, terpenoids, steroids, anthraquinones and alkaloids. The presence of these secondary metabolites in this plant might be responsible for the antitrypanosomal activity exhibited by its extracts.