In vitro and In vivo Anticonvulsant Effect of Hydroalcoholic Extracts of Clutia abyssinica in Mice Model
Samson Sahile Salile, Teferra Abula
Journal of Complementary and Alternative Medical Research · pp. 8–14 · Published 3 Apr 2021
10.9734/jocamr/2021/v13i430230Abstract
Background: Epilepsy is a chronic disorder of the brain that affects people of all ages worldwide. In the search of safe and effective antiepileptics traditional treatment practices are one area of research to obtain novel molecules. Research is also needed to validate and standardize the traditional claim. Clutia abyssinica leaves were one of the medicinal plants claimed for use against epilepsy and evil eye and other diseases in different parts of Ethiopia. But there was no scientific research evidence for the claimed use of the plant. Therefore this work was designed to evaluate the anticonvulsant effect of hydroalcoholic extract of Clutia abyssinica leaves. Methods: The dry residue of the plant extract was used for the test. In vitro 0Mg2+ mice model at dose 0.7 mg/kg of extract, diazepam (3μM) and untreated brain slice groups were used to compare the presence of seizure like event (SLE). In vivo pentylenetetrazol (PTZ) model with 85 mg/kg subcutaneously was used to compare the seizure onset time with two extract doses and diazepam 5 mg/kg. The data was presented with mean± standard error. In maximum electric shock (MES) model 54 mA was passed for 0.2 second transauricularly in mice. The mean time of hind limb extension was recorded for doses 400 mg/kg and 800mg/kg of the extract and 10 mg/kg phenytoin. The means were compared for statistical significance using one way ANOVA post hoc LSD whereas proportions were compared using Fishers exact test with P-value < .05. Results: In vitro anticonvulsant tests C. abyssinica extract effect was not statistically significant compared to negative control (P>0.05).A positive control using the known anticonvulsant diazepam (3μM), showed significant anticonvulsant activity (P<0.05). The in vivo PTZ test showed no statistically significant effect in plant extract at all dose levels (P>0.05). In the in vivo MES test the extract of Clutia abyssinica both low and higher dose didn't show statistically significant effect (P>0.05) compared with the negative control. But the extract improved survival (p<0.05). The qualitative secondary metabolite test evidenced the presence of alkaloids, cardiac glycosides, flavanoids, phenols, saponins, sterols and terpeoids in Clutia abyssinica extract. Conclusion: The hydroalcoholic crude extract result of the C. abyssinica as anticonvulsant is weak based on the models used in this study. For most of the local preparation are mixes of different plants it may have synergistic action with other plants. Or it may have action with other models of chronic epilepsy.
References (11)
- 1 Critical review of current animal models of seizures and epilepsy used in the discovery and development of new antiepileptic drugs [DOI]
- 2 Low extracellular magnesium induces epileptiform activity and spreading depression in rat hippocampal slices [DOI]
- 3 Key factors in the discovery and development of new antiepileptic drugs [DOI]
- 4 Regional and time dependent variations of low Mg2+ induced epileptiform activity in rat temporal cortex slices [DOI]
- 5 Ethnomedicinal survey of folk drugs used in Bahirdar Zuria district, Northwestern Ethiopia
- 6 Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes [DOI]
- 7 Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK 1‐ WG 4 report of the AES / ILAE Translational Task Force of the ILAE [DOI]
- 8 Evaluation of Hepatoprotective Activity of the Crude Extract and Solvent Fractions of Clutia abyssinica (Euphorbiaceae) Leaf Against CCl4-Induced Hepatotoxicity in Mice [DOI]
- 9 Evaluation of Anticonvulsant Actions of Dibromophenyl Enaminones Using In Vitro and In Vivo Seizure Models [DOI]
- 10 Assessment of the Indigenous Knowledge and Use of Traditional Medicinal Plants in Wolaita Zone, Southern Ethiopia [DOI]
- 11 In vitro and In vivo Anticonvulsant Effect of Hydroalcoholic Extract of Moringa stenopetala in Mice Models [DOI]
Cited by 1
Y. Birhan · Chinese Medicine · 2022
Related research
- Effect of Crude Ethanol Leaf-extract of Murraya koenigii on Anxiety in Mice — shares topic coverage
- Liver Profile of Artemether-lumefantrine-tinidazole in Healthy and Parasitized Mice — shares topic coverage
- Harnessing Ashitaba Extract: A Promising Solution for Regulating Blood Glucose in High Glucose Diet Mice — shares topic coverage
- Evaluation of Anti-malarial Profile of Methanol Leaf Extract of Jatropha curcas Linn on Plasmodium berghei Infection in Mice — shares topic coverage
- Histological Effect of Static Magnetic Fields on Testis and Epididymis in Male Mice — shares topic coverage
Article metrics
Real usage data collected on this platform.
14
Page views
0
PDF downloads
0
Outbound clicks
1
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.