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Research Article Open access CC BY 4.0

In-vitro and In-vivo Anti-Candidal Effect of Cnidoscolus aconitifolius Leaves Extracts

J. C. Adindu, C. O. Anyamene, C. U. Ezebialu, D. C. Chukwukaelo, C. F. Chude, L. E. Eruala, A. Umeofor

Journal of Advances in Microbiology · pp. 27–37 · Published 22 May 2023

10.9734/jamb/2023/v23i6729

Abstract

Candidiasis is an opportunistic infection caused by pathogens of the genus Candida. Due to the increase in antifungal resistance by Candida species, there is a need for alternatives in its treatment. Cnidoscolus aconitifolius is a medicinal plant with vast nutritional and antimicrobial properties. This study evaluated the in-vitro and in-vivo anti-candidal effect of Cnidoscolus aconitifolius (spinach tree) leaves extract. Clinical isolates of Candida albicans, Candida krusei, Candida tropicalis, Candida dublinensis, Candida glabrata, and Candida parapsilosis were used in this study and Cold maceration technique was employed in the plant extraction process, to obtain its ethanolic, methanolic, and aqueous extract. The agar well diffusion method and 2-fold dilution process -to obtain 250 mg/ml to 15.625mg/ml of the extracts -were used for the antimicrobial sensitivity test and three weeks old female Wistar rats infected with 20µl of standardized C. albicans were used for the in-vivo evaluation. Statistical analysis was done using Statistical package for Social Sciences (SPSS) version 22. In-vitro assay revealed ethanolic extract as the most potent extract with the highest inhibition zone diameter of 12.67±1.15c, 11.67±1.15c, and 12.33±0.58c at 250mg/ml against C. tropicalis, C. krusei2, and C. parapsilosis respectively. C. parapsilosis was the most sensitive to all the extracts. In-vivo, the disappearance of disease symptoms and progressive decrease in rats Candida burden- from 6.35×103 CFU/ml obtained after infection to 2.15×103CFU/ml after treatment revealed the ability of the plant to treat candidiasis. The study suggests that extract of Cnidoscolus aconitifolius leaves could act individually or synergistically as effective drug candidates in the treatment of candidiasis; thus if its individual antifungal constituents are purified, this plant could be the next major anti-candidal agent.

Candidiasis Cnidoscolus aconitifolius female wistar rats candida species anti-candidal effect

References (21)

  1. 1 District Laboratory Practice in Tropical Countries [DOI]
  2. 2 Antibiotic assay by agar-well diffusion method
  3. 3 Twenty Years of the SENTRY Antifungal Surveillance Program: Results for Candida Species From 1997–2016 [DOI]
  4. 4 Virulence Factors in Candida species [DOI]
  5. 5 Justification for the use of Ocimum gratissimum L in herbal medicine and its interaction with disc antibiotics [DOI]
  6. 6 Phytochemical and pharmacological activities of methanol extract of Artemisia vulgaris L. leaves [DOI]
  7. 7 Laboratory methods in antimicrobial chemotherapy
  8. 8 Efficacy of antifungal drugs in the treatment of oral candidiasis: A Bayesian network meta-analysis [DOI]
  9. 9 Laboratory Methods in Antimicrobial Chemotherapy [DOI]
  10. 10 Hepatoprotective Effects of Ethanolic Extract of Cnidoscolus aconitifolius on Paracetamol-Induced Hepatic Damage in Rats [DOI]
  11. 11 A New Model of Vaginal Infection by Candida albicans in Rats [DOI]
  12. 12 Cnidoscolus aconitifolius Leaf Extract Protects against Hepatic Damage Induced by Chronic Ethanol Administration in Wistar Rats [DOI]
  13. 13 Efficacy of Pterocarpus angolensis crude extracts against Candida krusei, Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli [DOI]
  14. 14 GC-MS Analysis of Cnidoscolus aconitifolius Leaf Aqueous Extracts [DOI]
  15. 15 The effect of Cnidoscolus aconitifolius on multi-drug resistant micro-organisms [DOI]
  16. 16 Antifungal Resistance Pattern of Candida Species Isolated from High Vaginal Swabs of Women Attending a Hospital in Enugu State, Nigeria [DOI]
  17. 17 The phytochemical and pharmacological screening of three crude extracts of Desmodium canum (strong back) [DOI]
  18. 18 International Journal of Science and Research (IJSR) [DOI]
  19. 19 Atlas of Medicinal Plants of Middle America: Bahamas to Yucatan
  20. 20 Natural product chemistry for drug discovery [DOI]
  21. 21 Energy Transition in West African Monetary Zone (WAMZ): Does Financial Development Matter? [DOI]

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