In silico Analysis of SlNAC1 Transcription Factor, a Positive Modulator for Tomato Leaf Curl Virus

Abstract

NAC proteins, part of the largest plant transcription factor family, play pivotal roles in plant development, and biotic and abiotic stresses. Among them, the SlNAC1 protein in tomato serves as a Negative regulator, enhancing tolerance to cold, bacterial, and viral diseases. However, our knowledge of SlNAC1 protein in silico analysis, evolutionary relationships, and protein interactions remains limited. We used in silico tools to study the SlNAC1 protein in tomato. Phylogenetic analysis showed it is closely related to other Solanaceae family members. Motif analysis revealed seven conserved motifs shared among Solanaceae members. The presence of NLS confirms its abundance in the nucleus. Protein-protein interaction analysis predicted ten potential nuclear partners, including five uncharacterized proteins and others involved in plant development. In silico expression, analysis showed abundant SlNAC1 presence in both fruit and roots. This study provides the role of SlNAC1 protein in tomato, shedding light on its evolutionary context and    protein interactions.