Impact of Various IBA Concentrations on Rooting in Apical Shoot Cutting and Stem Cuttings of Callistemon lanceolatus (Sm.) Sweet in Polytunnel Conditions

Abstract

The study was undertaken to identify the optimal concentration of IBA and type of cuttings suitable for propagation of Callistemon lanceolatus and it was conducted at the Vanavarayar Institute of Agriculture, Pollachi, Coimbatore, India from December 2022 to September 2023. The experiment addressed the challenges of seed propagation, such as their seed capsules need to be activated by fire and the unpredictable nature of seed germination, which results in high progeny diversity due to genetic recombination. The investigation focused on apical and stem cuttings subjected to fifteen different treatments with three replications each; the Tukey test was used for statistical analysis; the cuttings were treated with various concentrations of Indole-3-butyric acid (IBA) and placed under polytunnel conditions; observations were made after 45 days, measuring sprouting percentage, rooting percentage, mean number of leaves, shoot length, mean number of roots, and root length per cutting. In light of these issues, the study explored clonal propagation as an alternative for mass multiplication of elite trees. The results of the study depicted that the maximum rooting percentage and root length were observed in T6 (73.28±8.87%) and (12.54±0.00cm) due to the exogenous application of auxin. On the whole, the result of this investigation suggest that apical shoot cuttings with IBA at 6000ppm can be made to induce maximum rooting and to produce high quality planting stock material for clonal propagation in Callistemon lanceolatus.