Skip to content
Research Article Open access CC BY 4.0

Impact of Sacchromyce cerevisiae (Yeast) Supplementation in Varied Cereal-Based Broiler Starter Diets on Gut Microflora and Morphology

A. D. Ologhobo, Adighibe Lois Chidinma, O. O. Adeleye, I. O. Adejumo

Journal of Scientific Research and Reports · pp. 73–81 · Published 5 Feb 2015

10.9734/JSRR/2015/15810

Abstract

The experiment investigated the effect of yeast, Saccharomyces cerevisiae (SC) on gut microflora and gut morphology integrity of broiler chickens. A total number of 324 day old Arbor Acres broilers chicks were allocated to 9 x 3 replicates pens consisting of twelve birds each. One of the nine diets consisted of wheat, maize and sorghum with 5% SC inclusion each while the other six diets are the positive and negative control (positive control- maize, wheat and sorghum with antibiotics and negative control-without SC were fed for twenty-eight days. Data on gut morphology and gut microflora from the caeca were collected. At 28 days of age, two birds per replicate that is six birds per treatment were sacrificed through cervical dislocation. Sections of ileum were cut, flushed in distilled water and fixed in 10% formalin for 48hours. The tissues were grossed and cut into smaller pieces of 3mm thick in pre labeled tissue cassette. They were processed using Automatic tissue processor. Total anaerobic counts (TAB) were determined using Fastidious Anaerobe agar (FAA) incubated at 37°C for 5 days. Enterococci counts were enumerated using Slanetz and Bartley incubated aerobically at 37°C for 48 hrs. Coliform counts were enumerated using MacConkey agar, incubated aerobically at 37°C for 24 hrs and Lactobacilli counts were determined using de Man Rogosa and Sharpe (MRS) agar, incubated anaerobically at 38°C for 48 hrs. After incubation, colonies formed on the respective media were carefully counted and calculated. Data obtained were subjected to analysis of variance and means were separated using Duncan multiple range test. Inclusion of SC in the diets had a beneficial effect on the ileum morphology resulting in a significant increase (P<0.05) in villus height of birds fed maize +SC, wheat +SC, sorghum +SC, maize +AB and sorghum +AB but crypt depth, muscular wall length (MWL), villus to crypt ratio and goblet cells number were not significantly different in the dietary treatments. Although the highest crypt, MWL, villus/ crypt ratio and goblet cells number were recorded in Maize +AB (1526.8 µm), sorghum +AB (3466.9 µm), and wheat + SC (2750.7 µm and 129) respectively. Inclusion of SC in the diets had no significant difference on the ceca digesta of broiler birds compared to the positive and negative control on Coliform, total anaerobic bacteria and Lactobacillus sp. Populations. Significant difference (P<0.05) was observed in Enterococci population. Birds on sorghum -SC were significantly higher than birds on sorghum + AB but comparable with rest of the dietary treatments. Using Saccharomyces cerevisiae (SC) as a growth supplement can enhance the growth and nutrient absorption of broiler birds through the inhibitory effect of yeast against pathogenic microorganisms.

Saccharomyces cerevisae cereal grains gut microflora gut morphology

References (18)

  1. 1 The effects of dietary mannaoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of salmonella-challenged broiler chicks [DOI]
  2. 2 Effects of yeast (Saccharomyces cerevisiae) cell components on growth performance, meat quality, and ileal mucosa development of broiler chicks [DOI]
  3. 3 Probiotics in poultry: modes of action [DOI]
  4. 4 Effects of yeast culture supplementation on growth performance, intestinal health, and immune response of nursery pigs1 [DOI]
  5. 5 Effects of diets containing different concentrations of mannanoligosaccharide or antibiotics on growth performance, intestinal development, cecal and litter microbial populations, and carcass parameters of broilers [DOI]
  6. 6 Alternatives to Antibiotics for Organic Poultry Production [DOI]
  7. 7 Mucin Gene Expression and Mucin Content in the Chicken Intestinal Goblet Cells Are Affected by In Ovo Feeding of Carbohydrates [DOI]
  8. 8 Effects of Dietary Mannan Oligosaccharide, Bacitracin Methylene Disalicylate, or Both on the Live Performance and Intestinal Microbiology of Turkeys [DOI]
  9. 9 Prebiotics Modulate Immune Responses in the Gut-Associated Lymphoid Tissue of Chickens [DOI]
  10. 10 Mucin Dynamics in the Chick Small Intestine Are Altered by Starvation [DOI]
  11. 11 Exposure of different bacterial inocula to newborn chicken affects gut microbiota development and ileum gene expression [DOI]
  12. 12 Effect of mannan oligosaccharides on the performance, intestinal morphology and cecal fermentation of fattening rabbits [DOI]
  13. 13 The Effect of Mannanoligosaccharides, Bambermycins, and Virginiamycin on Performance of Large White Male Market Turkeys [DOI]
  14. 14 Pet food and feed applications of inulin, oligofructose and other oligosaccharides [DOI]
  15. 15 Chito-oligosaccharides as an Alternative to Antimicrobials in Improving Performance, Digestibility and Microbial Ecology of the Gut in Weanling Pigs [DOI]
  16. 16 Oligosaccharides with Beneficial Health Effects: A Japanese Perspective [DOI]
  17. 17 Effects of probiotics: alone and in a mixture of Biosacc® plus Zinc Bacitracin on the caecal microflora of Japanese quail [DOI]
  18. 18 Research Note: Virginiamycin and Monensin, Alone or in Combination, in Turkey Broiler Diets [DOI]

Cited by 4

Supplementing tributyrin to cottonseed protein concentrate‐based diets can improve growth performance, lipid metabolism and distal intestinal immunity in hybrid grouper ( Epinephelus fuscoguttatus  ♀ ×  Epinephelus lanceolatus  ♂)

Bin Yin, Hongyu Liu, Beiping Tan · Aquaculture Nutrition · 2021

Peptides from hypothetical proteins of Lactobacillus acidophilus induce IL-4 and IL-10

Isaac Oluseun Adejumo, Olufemi Adebukola Adebiyi · Biomedical Research and Therapy · 2025

Showing 2 of 4 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

70

Page views

0

PDF downloads

41

Outbound clicks

4

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.