Immobilization and Thermostability of Manganese Peroxidase from Trichoderma harzianum
Hamed M. El-Shora, Mohsen E. Ibrahim, Mohamed R. Elmekabaty
Microbiology Research Journal International · pp. 1–11 · Published 28 Aug 2017
10.9734/MRJI/2017/35754Abstract
The aim of the present investigation was to isolate Manganese peroxidase (MnP) (EC 1.11.1.13) from Trichoderma harzianum. The optimal pH and temperature for the enzyme production were 5.0 and 30°C. The best carbon and nitrogen sources were sucrose and yeast extract. The best production of MnP was formed at the 5th day of growth using 1 and 5 mM of Mn2+. The enzyme was immobilized on luffa, ceramic, alginate and chitosan and the percentages of immobilization were 40.1%, 15.9%, 82.1% and 91.3% respectively. The immobilized enzyme was expressed better stability at 50°C specially that immobilized at chitosan. Thus, the present results suggest that Trichoderma harzianum is a good candidate for production of manganese peroxidase which can be used in many biotechnological applications.
Cited by 11
Hamed M. El-Shora, Mohsen E. Ibrahim, Reyad M. El-Sharkawy, Mohamed R. Elmekabaty · Journal of Advances in Microbiology · 2017
Ashish Kumar, P. Arora · Frontiers in Environmental Science · 2022
K. Jankowska, J. Zdarta, Adam Grzywaczyk · 2021
H. El-Shora, R. El-Sharkawy · Journal of General and Applied Microbiology · 2020
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