α-glucosidase (EC: 3.2.1.20) from Penicillium chrysogenum Thom ATCC 10106 was induced by GSH at the lower concentrations. H2O2 was inhibitor at all tested concentrations and the IC50 was 92.2%v/v. AMP, ADP and ATP enhanced the activity revealing that α-glucosidase is endothermic...
Open access
Research Article10.9734/ARRB/2018/38408
This research aimed to purify and immobilize chitinase from Alternaria infectoria and to study its biochemical characteristics for biotechnological applications. Chitinase (EC. 3. 2. 1. 14) was purified by ammonium sulphate precipitation, DEAE-cellulose and then Sephadex G-200 wi...
Open access
Research Article10.9734/MRJI/2017/36263
Manganese peroxidase (MnP) was isolated and purified from T. harzianum. The free and immobilized enzyme was activated by thioglycolate and reduced glutathione (GSH). After treatment with thioglycolate, the Vmax values for the free, alginate-immobilized MnP (alginate-E) and chitos...
Open access
Research Article10.9734/JAMB/2017/36798
The aim of the present investigation was to isolate Manganese peroxidase (MnP) (EC 1.11.1.13) from Trichoderma harzianum. The optimal pH and temperature for the enzyme production were 5.0 and 30°C. The best carbon and nitrogen sources were sucrose and yeast extract. The best prod...
Open access
Research Article10.9734/MRJI/2017/35754
Zygophyllum coccineum L. (Family Zygophyllaceae) was collected from two locations namely Wadi Hagul and Deltaic Mediterranean coast. Methanol, acetone and ethanol extracts prepared from Z. coccineum leaves and they were tested for their antibacterial activity. The bacterial strai...
Open access
Research Article10.9734/BJAST/2016/24541
Zygophyllum coccineum L. (Family Zygophyllaceae) was collected from two habitats namely Wadi Hagul and Nile Delta coast. Plant leaves from both habitats contained appreciable content of total phenols and total flavonoids. The contents of these compounds were remarkably higher in...
Open access
Research Article10.9734/BJAST/2016/24540