Hyphal Formation is Enhanced by 121°C Heat Inactivation of Serum Added to Culture Media
Hong He, Xueting Wang, Congcong Li, Xiaotong Deng, Yan Fan, Jianxin Han
Journal of Advances in Medicine and Medical Research · pp. 12–20 · Published 2 May 2020
10.9734/jammr/2020/v32i630427Abstract
Background: A novel method for inducing hyphae formation, was described. Methods: Mycelia of experimental and clinical strains from 100 patients with oral lichen planus were cultured in RPMI 1640 medium with 56°C or 121°C inactivated calf sera. After these strains were cultured for 2 h to 7 days, the average hyphae formation rate was observed under light microscopy. Results: The 121°C inactivated calf serum showed better outcomes than 56°C inactivated calf serum in terms of time and amounts of hyphae products. Conclusions: For Candida albicans hyphae culture, the 121°C inactivated serum is superior to the 56°C inactivated serum.
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