Antioxidant Activity of nHexane Extract of Caryota no Seed Using Drosophila melanogaster Model

Abstract

Aims: To investigate the anti-oxidant activity of the nhexane extracts of Caryota no seeds in D. melanogaster. Study Design: Experimental design. Place and Duration: Sample: African Centre of Excellence for Phytomedicine Research and Development, University of Jos, Jos Plateau State Nigeria between June 2018 and February 2019. Methodology: Total protein assays were carried out by exposing 50 flies in each vial to the following concentrations: 300 mg, 350 mg, 400 mg, 500 mg and 600 mg of nhexane extracts in 5 replicates for 7 days with daily recording of mortality. In vivo antioxidant activity study was conducted by measuring levels of Glutathione-S-Transferase (GST), catalase (CAT) and total thiol (TT) from supernatants of whole fly homogenates using a spectrophotometer at specific wavelenghts. The values were derived as part of the total protein value. The statistical difference among test groups was presumed at P <0.05.                                    Results: The nhexane extract of CN caused nonsignificant (P = .52) changes in total protein levels compared to the control. The antioxidant activity showed nonsignificant (P = .64) higher total thiol contents and GST (P = .76) activity in nhexane extract-treated flies and lower catalase (P = .30) activity was recorded in the nhexane extract-treated flies compared to the controls. Conclusion: It can therefore be concluded that the nhexane extract of Caryota no possess appreciable concentrations of different types of antioxidants.