Isolation, Characterization and Protein Profiling of Lead Resistant Bacteria
Sabyasachi Chatterjee, Juthika Das, Saheli Chatterjee, Pratiti Choudhuri, Agniswar Sarkar
Microbiology Research Journal International · pp. 116–131 · Published 16 Oct 2013
10.9734/BMRJ/2014/5862Abstract
Aims: To isolate lead resistant bacteria from industrial effluent and characterize them by biochemical tests, effect of physical and chemicals factors on their growth and protein profiling of the isolates on with or without metal stress. Study Design: Cross-sectional study of related research articles and papers. Place and Duration of Study: Samples were collected from effluent water of Indian Iron and Steel Company (IISCO), Burnpur, West Bengal, India and studied at The Department of Biotechnology, The University of Burdwan. West Bengal, India, between September 2010 and June 2011. Methodology: Isolation of the isolates was done by pour plating the diluted effluent water onto the metal (Lead acetate) containing agar plates. The basic characterization of the isolates was done on the basis of morphological, physical, biochemical and antibiogram tests. Quantification of metal absorption was determined by Atomic absorption Spectrophotometric analysis. Protein expressions of the isolates on metal stress were studied by SDS-PAGE. Results: Two bacterial isolates namely PbrB1 and PbrB2 were isolated; they showed resistance to lead up to 6mM Lead (Lead acetate) and also to different antibiotics. They are able to ferment different sugars even in presence of low concentration of metals. Their protein profiling by SDS-PAGE shows different expressions of proteins upon metal stress. Conclusion: The isolates can be utilized to bioremediate lead from contaminated environment and further study of molecular mechanisms underlying lead accumulation process can be studied.
Cited by 11
Simran Yadav, Rajnish Kajala, Deepak Rajpurohit · Annals of Microbiology · 2025
M. Mandal, S. Das, S. Mandal · Access Microbiology · 2020
P. Singh, Anamika Kushwaha, Nidhi Hans · Brazilian Journal of Microbiology · 2019
M. Bose, S. Datta, P. Bhattacharya · 2017
Hassan A. H. Ibrahim, Hanan M. Abd Elnaby, A. Farag · 2014
Pushpendra Singh, A. Chopra · 2014
A. Sugasini, K. Rajagopal · 2014
Pushpendra Singh, A. Chopra · 2014
A. H. Ibrahi · 2014
Aakanksha Chourasia, Rupesh Dutta Banik, Pritha Pal · Bioremediation Journal · 2025
Related research
- Identification, Comparison, and Transfer of the pxo Gene between Members of Bacilli Species — shares topic coverage
- Production and Characterization of a Thermostable and Ca++ Independent Amylase Enzyme from Soil Bacteria — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
11
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.