Formulation of Silver Nanoparticles from Grewia Asiatica Leaf Extract and their Biomedical Applications

Abstract

Nanotechnology is a well-advanced area and several types of nanoparticles have been synthesized. Different types of nanoparticles have been synthesized so far using different techniques. It is most practical to produce nanoparticles from plant extracts for use in medicine and other applications. In this study, silver nanoparticles were synthesized by Grewia asiatica leaf extract. Synthesized nanoparticles were characterized by different techniques including Ultraviolet-visible (UV) spectroscopy, X-ray diffraction (XRD), Dynamic light scattering (DLS), and Scanning electron microscopy (SEM). Results showed a characteristic peak of silver nanoparticles in UV-region at 425nm, while DLS analysis revealed the z-average size of synthesized particles i.e., 461.6nm and -28.4mV zeta potential. SEM analysis revealed the amorphous surface morphology. The antibacterial and antioxidant activity of silver nanoparticles were also evaluated. Silver nanoparticles showed significant antibacterial activity against both gram-positive and gram-negative bacteria. A maximum zone of inhibition of 16mm diameter was observed against Bacillus subtilis and Klebsiella pneumonia. While results of antioxidant activity showed a 43.27μg/ml IC50 value. In conclusion, Grewia asiatica leaf extract is a good source to synthesize silver nanoparticles. Also, green synthesized silver nanoparticles could act as potential antibacterial and antioxidant agents.