The Effect of Endosulfan on Ovaries of Mus musculus: A Histological Study

Abstract

Background: Today pesticidal poisoning is an important health issue. The term “pesticides” covers a wide range of substances of differing chemical composition. Although we know these are useful in agricultural sector. However, it’s increased and unprotected use in agricultural practices has greatly enhanced the chances of its exposure to animals and man. This increases the risk of health disorders. Objective: The aim of present study was designed to examine the effect of pesticide an Endosulfan on the ovary of female Mus musculus. Materials and Methods: They were administered orally according to their body weight with Endosulfan @ 3 mg/kg body weight for 2 weeks, 4 weeks, 6 weeks and 12 weeks. The animals were sacrificed on day next to the last day of treatment and ovaries were extirpated. Ovaries were studied histologically after haematoxylin - eosin staining. Results and Discussion: Observations showed structural defects during histological examinations of Graafian follicles after exposure of Endosulfan. Degeneration in follicular cells was seen and ova were often detached. Degeneration of ova occured in some follicles, irrespective of the stage of their development. The organization of granulosa cells was also disturbed in longer duration of treatment. The abnormalities gradually increased with dose duration time. Such types of abnormalities have not been seen in the control ovary. Conclusion: It may be concluded from this study, Endosulfan has adverse effects on ovary at cellular level. Damaged cells of ovarian follicles, destroys steroid hormone production, which can result into improper ovarian function.