Effects of Bacteriocin Extracted from Lactobacillus rhamnosus on Cutaneous Wound Healing in Mice

Abstract

Aim: This study evaluates the effects of bacteriocin produced by Lactobacillus rhamnosus on cutaneous wound healing in mice. Study Design: Randomized experimental design was employed in the study from June-September, 2022. Methodology: Probiotic L. rhamnosus was isolated from nono milk using MRS agar media and identified based on morphological and biochemical characteristics. The potent bacterium was subjected to bacteriocin production. Antimicrobial activity of the bacteriocin was carried out against wound pathogenic bacteria and wound healing effects of the bacteriocin was assessed on mice. 6 mm cutaneous wound was induced on the mice and crude bacteriocin was topically applied on the wounds. White blood cell count was carried out after days 3, 5, 9, and 14. Histopathological analyses were performed after day 14. Results: Crude bacteriocin was effective against wound pathogenic bacteria such as S. aureus, P. aeruginosa and E. coli. Topical application of bacteriocin showed a faster and better wound healing when compared to positive control groups in P. aeruginosa infected wound model. Histology examination revealed an increase wound healing time in L. rhamnosus bacteriocin treated mice. The crude bacteriocin demonstrated excellent wound-healing activity in P. aeruginosa infected wound model.  Conclusion: L. rhamnosus bacteriocin had various beneficial actions on wounds. It improved wound healing, inhibited wound pathogens and increase wound healing time.