Green Synthesis of Copper Nanoparticles Using Stem Extract of Musa sapientum and Its Characterisation: an in vitro Study

Abstract

Introduction: Metallic nanoparticles (NPs) have attracted great interest because of their unique physical and chemical properties and among which copper nanoparticles are used for its low cost, better efficacy and environmentally friendly. Musa sapientum has been considered as one of the best medicinal value plants and can be used for the treatment of various periodontal diseases. The aim of the study is to synthesize and characterize Musa sapientum mediated copper nanoparticles. Materials and Methods: The Copper nanoparticles (CuNPs) were synthesized using Musa sapientum extract and characterization was done by UV-Visible Spectroscopy and Transmission Electron Microscope (TEM). Results: The results confirmed that the synthesized Musa sapientum mediated nanoparticle was eco-friendly and non-toxic. The peak value has been centered at 75 nm, which has been mainly associated with absorbance of CuNPs. Absorbance rate increases with increase in wavelength. The formation of CuNPs as well as their morphological dimensions in the TEM study demonstrate that the average size is from 5.67 – 9.10 nm. The shapes of the CuNPs are proved to be spherical. TEM analysis reveals that the copper nanoparticles are predominantly spherical. The overall morphology of the copper nanoparticles produced by reduction of cu2+ ions with 2Mm CUSO4 is composed of almost uniform nanoparticles. Conclusion: In this study, a simple, biological and low-cost approach was done for the preparation of copper nanoparticles using stem extract of Musa sapientum. Thus Musa sapientum mediated copper nanoparticles can be subjected to the various other biological activities such as antibacterial, antifungal, cytotoxic evaluation to know the efficiency of these nanoparticles.