Green Synthesis of Copper Nanoparticles Using Ginger Oleoresin and Evaluation of its Anticancer Activity against Liver Cancer Cell Line

Abstract

Introduction: Nanotechnology is getting used in developing countries to assist, treat disease, and stop health issues. Nanotechnology in addition to nanomedicine is being applied to or developed for application to a spread of commercial and purification processes. Ginger, scientifically known as Zingiber officinale, belongs to the family Zingiberaceae. Aim: The aim of the study was to green synthesis copper nanoparticles using ginger oleoresin and to evaluate its cytotoxic activity against liver cancer cells. Materials and Methods: The Ginger oleoresin was obtained from Synthite Industries Private Limited, Kerala with a product code: 4010000370 was used for the study. Copper nanoparticles were prepared from oleoresin and confirmed using UV-Visible spectroscopy. The prepared copper nanoparticles were then evaluated for the anticancer effect on liver cancer cells using the method of  Mosmann. The cells (1 × 105 cells per ml) were seeded in a 96 well microtiter plate (100 μl per well) with replications. Different concentration  (control, 10, 20, 30, 40, and 60 μg/ml) of ginger oleoresins mediated copper nanoparticles were tested for the anticancer activity. The percentage of cell viability was calculated. Results: Dose-dependent anticancer activity was observed with ginger oleoresins mediated copper Nanoparticles. The IC 50 value  was 30 mg / ml Conclusion: In the present study, ginger oleoresin showed a good activity cytotoxic effect on liver cancer cell lines. However, more research is needed to understand the underlying mechanisms of the cytotoxic property of the ginger plant.