Phenotypical Characterization of Pseudomonas aeruginosa Clinical Strains Isolated in Abidjan (Côte d'Ivoire)

Abstract

Aims: Objective of this study was to determine the prevalence of clinical Pseudomonas aeruginosa strains expressing antibiotic resistance mechanisms of the family of β-lactams, aminoglycosides and fluoroquinolones in Abidjan. Study Design: It is a retrospective study. Place of Study: Laboratories of Bacteriology and virology of Pasteur Institut, Abidjan, Côte d'Ivoire. Methodology: From January 2012 to December 2015 of P. aeruginosa strains from various hospital departments and pathological products of patients have been identified using conventional methods of Bacteriology and Maldi-Tof. Susceptibility strain to antibiotics was tested using the standard Mueller-Hinton agar diffusion susceptibility test method according to CA-SFM 2015. Results: Two hundred and eight Pseudomonas aeruginosa strains were isolated which 35.83% coming from service of medicine. Among these strains 46.63% were isolated from abscess and 9.62% from urine. Antibiotics resistance profile showed a resistance of preferably β-lactams especially to ticarcillin (30.77%). Colistin was the most active antibiotic. Different types of phenotypes have been identified. Regarding β-lactams, the most observed phenotype were the wild ones with 61.06% of all the strains and 12.02% for the efflux phenotype. For aminoglycosides 69.23% were wild-type and 75.96% were wild type for fluoroquinolones.