In vitro Antiglycation, Antioxidant Properties of Coleus forskohlii ''Balady'' Leaves and Stem and their Antioxidant Enzyme Activities

Abstract

Aims: In this study, the antiglycation and antioxidant potency of methanol (80%) extracts, as well as the antioxidant enzyme activities from both leaves and stem of Coleus forskohlii “balady”, were analyzed. Place and Duration of Study: Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia, between February 2017 and July 2017. Methodology: Antiglycation activity was evaluated by BSA-glucose glycation assay. The total phenolics, flavonoids, chlorophyll, carotenoid, lycopene content were estimated, and antioxidant activity was tested by the capacity to reduce ABTS reducing power assay, DPPH radical-scavenging activity assay, FRAP assay. Enzyme activities from both leaves and stem of Coleus forskohlii were tested for polyphenol oxidase, peroxidase, and catalase. Results: Results indicated that methanolic extracts from leaves exhibited strong antiglycation and antioxidant activities than from stem. Among the enzymatic antioxidant properties, except for the activity of peroxidase, the activities of catalase and polyphenol oxidase were significantly higher (P < 0.05) in leaves than in stem. Conclusion: It can be concluded that the leaves contained significantly rich sources of both enzymatic and non-enzymatic antioxidants can be used as a natural antioxidants.