Genetic Identification of Soybean Segregants Null for Kunitz Trypsin Inhibitor (KTI) and Lipoxygenase-2 (LOX2)

Abstract

The present investigation entitled was conducted at the Post Graduate Research Farm, Mahatma Phule Krishi Vidyapeeth, Rahuri and State Level Biotechnology Centre, Rahuri – 413 722 during the academic year 2024-25. The objectives included in this study was to depict the molecular markers linked to the quality traits viz., KTI and LOX2 and identify desirable segregants in soybean. A total of 100 F2 segregants 50 each from Cross I (JS 22 12 × NRC 142) and Cross II (JS 22 16 × NRC 142) were evaluated using three quality trait markers viz., titi gene specific was linked to KTI locus while remaining two, lox2 gene specific and Satt 656 linked to Lox-2 gene locus. The markers were used to identify segregants free from KTI and LOX2 genes. Out of 100 F2 segregants, 11 (#3, #5, #6, #8, #9, #15, #17, #20, #22, #38, #41) segregants from Cross I and 7 (#3, #9, #16, #26, #41, #44, #47) segregants from Cross II (JS 22 16 × NRC 142) were identified as free from KTI and LOX2 factors. These identified segregants were subsequently advanced to develop homozygous lines exhibiting null activity for both KTI and LOX2.