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Research Article Open access CC BY 4.0

Genetic and Kinetic Profiles of Bacterial Resistance Enzymes to IMP and NDM Type Antibiotics in Gram-negative Pathogenic Bacilli

Lionel Eliada Benoit Bambara, Amana Metuor Dabire, Oumar Traore, Yasmine Tiemtore, Theodora Zohoncon, Jacques Simporé

International Journal of Biochemistry Research & Review · pp. 7–21 · Published 16 Aug 2023

10.9734/ijbcrr/2023/v32i6819

Abstract

Introduction: Enterobacteria are among the most frequent isolated strains in hospitalized patients. Currently, carbapenems have a broad spectrum of activity and are considered to be the last resort for the treatment of nosocomial infections. However, overuse of these valuable compounds has led to a rapid increase in antimicrobial resistance. The aim was to study the genetic and kinetic profiles of IMP and NDM bacterial resistance enzymes in Gram-negative pathogenic bacilli. Methodology: The carbapenems used for strain susceptibility testing consisted of Ertapenem, Meropenem, Imipenem and Doripenem. Detection of the IMP and NDM resistance genes encoding carbapenemases was carried out by conventional PCR at LABIOGENE, and hydrolysis of the enzymatic crude extracts was performed. Results: Strains showed high levels of resistance to Ertapenem (64%), Meropenem (41%), Imipenem (53%) and Doripenem (73%). PCR amplification of carbapenem resistance genes showed that 32% of strains carried blaIMP, 16% of strains carried blaNDM. Both blaIMP & blaNDM were identified with a prevalence of 6.76%. Escherichia coli was the majority species harboring these genes. The kinetic constants of NDM carried by E. coli are Km=333µM and Vmax=0.5 A/min. Those of IMP carried by Klebsiella sp are Km=500µM and Vmax=0.6 A/min. Conclusion: This study enabled us to determine the rate of carbapenem resistance in Gram-negative bacteria. We noticed that Meropenem was the most active molecule. The study also enabled us to determine the affinity of two different bacterial enzymes to nitrocefin.

BGN carbapenem carbapenemases blaVIM blaNDM

References (16)

  1. 1 A Brief History of the Antibiotic Era: Lessons Learned and Challenges for the Future [DOI]
  2. 2 Natural product discovery: past, present, and future [DOI]
  3. 3 Resistance in gram-negative bacteria: Enterobacteriaceae [DOI]
  4. 4 Prospects for new antibiotics: a molecule-centered perspective [DOI]
  5. 5 The etiology of urinary tract infection: Traditional and emerging pathogens [DOI]
  6. 6 Carbapenemase Genes among Multidrug Resistant Gram Negative Clinical Isolates from a Tertiary Hospital in Mwanza, Tanzania [DOI]
  7. 7 High Levels of Extended-Spectrum Beta-Lactamases in a Major Teaching Hospital in Ghana: The Need for Regular Monitoring and Evaluation of Antibiotic Resistance [DOI]
  8. 8 The resistance tsunami, antimicrobial stewardship, and the golden age of microbiology [DOI]
  9. 9 Epidemiology of infections caused by multiresistant gram-negatives: ESBLs, MBLs, panresistant strains.
  10. 10 Prevalence of metallo-β-lactamase acquired genes among carbapenems susceptible and resistant Gram-negative clinical isolates using multiplex PCR, Khartoum hospitals, Khartoum Sudan [DOI]
  11. 11 Detection of VIM and NDM-1 metallo-beta-lactamase genes in carbapenem-resistant Pseudomonas aeruginosa clinical strains in Bahrain [DOI]
  12. 12 Multidrug-resistant Enterobacteriaceae nosocomial uropathogens at Menoufia University Hospitals: phenotypic characterization and detection of resistance genes using real-time PCR [DOI]
  13. 13 Isoelectric focusing of β-lactamases [DOI]
  14. 14 Comparison of routine techniques for cell breakage and release of β-lactamase activity [DOI]
  15. 15 Agarose Gel Electrophoresis [DOI]
  16. 16 Detection of NDM-1, VIM-1 and AIM-type metallo-beta-lactamase genes in Gram-negative bacteria isolated from clinical samples in Tamil Nadu [DOI]

Cited by 6

Coexistence of imipenemase (IMP) and verona integron-encoded metallo-beta-lactamase (VIM) in gram-negative bacterial clinical isolates from Ouagadougou, Burkina Faso

Amana Metuor-Dabiré, Damis Y. P. Bouniounou, Nicolas Ouédraogo · Microbiology Independent Research Journal (MIR Journal) · 2025

Multi-resistance to carbapenems by the production of Imipenemase (IMP)-types carbapenemases in Gram-negative bacilli in Burkina Faso

Yves Patrik Bouniounou Damis, Metuor Dabire Amana, Yasmine Tiemtore Rahimatou · African Journal of Biotechnology · 2024

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