Isolation and Identification of Bordetella bronchiseptica from Clinical Respiratory Tract Infections of Canines in Mizoram: First Report

Abstract

Bordetella bronchiseptica is one of the major bacterial causes of Canine Infectious Respiratory Disease Complex (CIRDC) with zoonotic potential. This research was designed to give information on the detection of B. bronchiseptica in presented canine cases at the Veterinary Clinical Complex. Oro-nasal and pharyngeal swabs were collected from 113 from canine cases suspected for respiratory tract infection. Detection was done by culture, isolation and identification, biochemical test and confirmatory diagnosis by Polymerase Chain Reaction. Luria Bertani broth was used for the pre-enrichment and Smith-Baskerville media were used for isolating B. bronchiseptica. The organism was grown in MacConkey agar and isolated using specific medium (Smith-Baskerville). Isolates on MacConkey agar showed non-lactose fermenting small pale colonies. The isolated organisms were positive for citrate, urease, catalase, and oxidase test and negative for sucrose and glucose fermentation test. The confirmation was done using PCR with published primers targeting fla gene. Samples from 12 canines were positive for the genus specific amplicon size (237 bp) of B. bronchiseptica. As the presence of the agent is confirmed in many research works, immunization of the puppies can be considered.